Theoretical and Applied Veterinary Medicine (Jun 2020)
The effect of a new feed additive of humic nature on metabolic processes in laboratory rats depending on the dose
Abstract
The effectiveness of the new humic nature feed additive – «Animal Forte», has been established, depending on its amount on the metabolism of laboratory rats. We used sexually mature male white rats of 2 months age, from which five experimental groups were formed and one intact, with eight animals each. The experiment lasted four weeks, during which the animals of the experimental groups received daily in addition to the main feed 5, 10, 20, 30 and 50 mg of feed additive with its active substance per 1 kg of animal’s weight. Morpho-functional indicators (hemoglobin content, hematocrit, red blood cells, platelets, and leukocytes) were determined in EDTA stabilized blood. To define the effectiveness of a new humic nature food supplement, the content of total protein, albumin and globulins, uric acid, urea, creatinine, glucose, and the activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT) were measured in blood serum. Red blood cell coefficients were determined by a calculation method: mean erythrocyte volume (MCV); mean erythrocyte hemoglobin content (MCH); mean hemoglobin concentration in erythrocyte (MCHC). A feed supplement at a dose of 10 mg/kg had a greater effect on hematopoietic function, especially on the erythropoiesis processes, namely, the hemoglobin content, the number of RBC, the RBC index – MCV, MCH were increased. Due to this, respiration and oxidation-reduction processes in animal tissue were improved. Usage of a biologically active humic nature substance at a dose of 5 mg/kg of animals weight to a greater extent compared with other options improved the function of liver hepatocytes, increasing its protein-synthesizing property, due to this effect, the total protein and albumin content in the serum of experimental animals were increased. Under the influence of the feed additive on metabolic processes, including the membrane’s permeability, in the blood of rats of experimental groups, the level of glucose was decreasing. The positive effect of Animal Forte on the kidneys filtration and excretory properties was also noted, due to which the content of urea and creatinine in the blood of rats was decreased. It has been established that already with a minimal dose of 5 mg/kg of body weight, the feed additive had an effect on animals’ metabolic processes.
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