Cancer Management and Research (Oct 2020)
miR-302e Suppresses Glioma Progression by Targeting VEGFA
Abstract
Yunpeng Xie,1 Xin Liu,2 Tiemin Hu,1 Weixing Wang1 1Department of Neurosurgery, Chengde Medical College Affiliated Hospital, Chengde, Hebei Province, People’s Republic of China; 2Department of Oncology, Chengde Medical College Affiliated Hospital, Chengde, Hebei Province, People’s Republic of ChinaCorrespondence: Weixing WangDepartment of Neurosurgery, Chengde Medical College Affiliated Hospital, No. 36, Nanyingzi Avenue, Chengde, Hebei Province, People’s Republic of ChinaEmail [email protected]: MiRNA can be involved in regulating tumor genesis and development by regulating the expression of specific genes and regulating corresponding signaling pathways. In this study, we explored the function and mechanisms of miR-302e in glioma progression.Methods: Experimental methods include the following: real-time quantitative PCR, Western Blot Analysis, CCK8 assay and detection of apoptosis.Results: MiR-302e was down-regulated in cancer tissues and cell lines, and the expression of miR-302e was negatively correlated with the tumor grade, which indicated poor prognosis in glioma patients. Followed functional analysis showed overexpression of miR-302e inhibited proliferation, migration and invasion but promoted apoptosis of glioma cells, while silencing miR-302e showed the opposite effects. Mechanistic studies have shown that VEGFA was a directed target of miR-302e. Forced expression of VEGFA removed the inhibiting impact of miR-302e on glioma development. In vivo tumorigenesis experiments showed that miR-302e suppressed glioma development by targeting VEGFA.Conclusion: Present study emphasized miR-302e suppressed glioma development by targeting VEGFA, which might be a valuable target for glioma treatment.Keywords: glioma, miR-302e, proliferation, migration, VEGFA
