Jichu yixue yu linchuang (Jun 2021)
Etomidate alleviates LPS-induced rat myocardial cell line H9C2 injury by upregulating miR-290-5p
Abstract
Objective To investigate the protective mechanism of etomidate (Eto) on lipopolysaccharide (LPS) induced myocardial injury. Methods H9C2 cells were divided into control (Co) group, LPS group (1 μg/mL LPS treatment for 6 h), Eto+LPS group, miR-con+LPS group, miR-290-5p+LPS group, Eto+anti-miR-con+LPS group, and Eto+anti-miR-290-5p+ LPS group. Tetramethylazozolium blue (MTT) method and flow cytometry were used to detect cell viability and apoptosis; real-time fluorescent quantitative PCR (RT-qPCR) was used to detect miR-290-5p expression. Enzyme-linked immunosorbent assay (ELISA) was used to detect tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) in cell culture fluid. Results Compared to the control group, the expression of miR-290-5p in H9C2 cells in the LPS group reduced (P＜0.05), the cell survival rate reduced(P＜0.05), the apoptosis rate increased (P＜0.05), and the level of TNF-α and IL-6 significantly increased (P＜0.05); compared with the LPS group, the expression of miR-290-5p in H9C2 cells in the Eto+LPS group was increased (P＜0.05), and the cell survival rate was decreased (P＜0.05), apoptotic rate was decreased (P＜0.05), TNF-α and IL-6 content were decreased (P＜0.05); compared with the miR-con+LPS group, the survival rate of H9C2 cells in the miR-290-5p+LPS group increased (P＜0.05), the apoptosis rate reduced (P＜0.05), the level of TNF-α and IL-6 reduced (P＜0.05); when compared with the Eto+anti-miR-con+LPS group, the survival rate of H9C2 cells in the Eto+anti-miR-290-5p+LPS group decreased(P＜0.05), and apoptosis rate increased (P＜0.05), the level of TNF-α and IL-6 increased(P＜0.05). Conclusions Etomidate reduces LPS-induced myocardial cell apoptosis and inflammatory response by up-regulating miR-290-5p.
