Laser Fabrication of 3D Gelatin Scaffolds for the Generation of Bioartificial Tissues

Abstract

In the present work, the two-photon polymerization (2PP) technique was applied to develop precisely defined biodegradable 3D tissue engineering scaffolds. The scaffolds were fabricated via photopolymerization of gelatin modified with methacrylamide moieties. The results indicate that the gelatin derivative (GelMod) preserves its enzymatic degradation capability after photopolymerization. In addition, the developed scaffolds using 2PP support primary adipose-derived stem cell (ASC) adhesion, proliferation and differentiation into the anticipated lineage.

Keywords

• scaffolds
• computer-aided design
• laser fabrication
• two-photon polymerization
• gelatin
• tissue engineering
• biodegradation
• stem cells
• adipose tissue