Revista da Sociedade Brasileira de Medicina Tropical (Apr 2015)

Production of metallo-β-lactamase among Pseudomonas aeruginosa strains isolated in the State of Sergipe, Brazil

  • Lívia Maria do Amorim Costa,
  • Maria Emília de Castro Kling Fleming,
  • Geraldo Renato de Paula,
  • Lenise Arneiro Teixeira,
  • Pedro Juan José Mondino,
  • Sílvia Susana Bona de Mondino,
  • Cláudia Rezende de Vieira Mendonça-Souza

DOI
https://doi.org/10.1590/0037-8682-0198-2014
Journal volume & issue
Vol. 48, no. 2
pp. 212 – 215

Abstract

Read online

INTRODUCTION: Acquired production of metallo-β-lactamases is an important mechanism of resistance in Pseudomonas aeruginosa. The objective of this study was to investigate the production of metallo-β-lactamase and the genetic diversity among ceftazidime-resistant P. aeruginosa isolates from State of Sergipe, Brazil. METHODS: Metallo-β-lactamase was investigated using the disk approximation test and polymerase chain reaction (PCR). Genetic diversity was evaluated by pulsed-field gel electrophoresis (PFGE). RESULTS: A total of 48 (51.6%) isolates were resistant to ceftazidime. Six (12.2%) of these were positive for metallo-β-lactamase production. Only two (4.1%) of the ceftazidime-resistant isolates carried the bla SPM-1 gene. CONCLUSIONS: Production of metallo-β-lactamases was not the main mechanism of resistance to ceftazidime and carbapenems among P. aeruginosa strains in Sergipe, Brazil.

Keywords