Frontiers in Molecular Biosciences (Jun 2023)
The platelet transcriptome and proteome in Alzheimer’s disease and aging: an exploratory cross-sectional study
- Diana M. Bessa de Sousa,
- Diana M. Bessa de Sousa,
- Rodolphe Poupardin,
- Rodolphe Poupardin,
- Saul A. Villeda,
- Adam B. Schroer,
- Thomas Fröhlich,
- Vanessa Frey,
- Vanessa Frey,
- Wolfgang Staffen,
- Heike Mrowetz,
- Heike Mrowetz,
- Barbara Altendorfer,
- Barbara Altendorfer,
- Michael S. Unger,
- Michael S. Unger,
- Bernhard Iglseder,
- Bernhard Paulweber,
- Eugen Trinka,
- Eugen Trinka,
- Eugen Trinka,
- Janne Cadamuro,
- Martin Drerup,
- Katharina Schallmoser,
- Katharina Schallmoser,
- Ludwig Aigner,
- Ludwig Aigner,
- Ludwig Aigner,
- Kathrin M. Kniewallner,
- Kathrin M. Kniewallner
Affiliations
- Diana M. Bessa de Sousa
- Institute of Molecular Regenerative Medicine, Paracelsus Medical University, Salzburg, Austria
- Diana M. Bessa de Sousa
- Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg, Austria
- Rodolphe Poupardin
- Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg, Austria
- Rodolphe Poupardin
- Experimental and Clinical Cell Therapy Institute, Paracelsus Medical University, Salzburg, Austria
- Saul A. Villeda
- Department of Anatomy, University of California San Francisco, San Francisco, CA, United States
- Adam B. Schroer
- Department of Anatomy, University of California San Francisco, San Francisco, CA, United States
- Thomas Fröhlich
- Laboratory of Functional Genome Analysis (LAFUGA), Gene Center, Ludwig Maximilian University of Munich, Munich, Germany
- Vanessa Frey
- Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg, Austria
- Vanessa Frey
- Department of Neurology, Christian Doppler Clinic, Paracelsus Medical University, Salzburg, Austria
- Wolfgang Staffen
- Department of Neurology, Christian Doppler Clinic, Paracelsus Medical University, Salzburg, Austria
- Heike Mrowetz
- Institute of Molecular Regenerative Medicine, Paracelsus Medical University, Salzburg, Austria
- Heike Mrowetz
- Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg, Austria
- Barbara Altendorfer
- Institute of Molecular Regenerative Medicine, Paracelsus Medical University, Salzburg, Austria
- Barbara Altendorfer
- Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg, Austria
- Michael S. Unger
- Institute of Molecular Regenerative Medicine, Paracelsus Medical University, Salzburg, Austria
- Michael S. Unger
- Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg, Austria
- Bernhard Iglseder
- Department of Neurology, Christian Doppler Clinic, Paracelsus Medical University, Salzburg, Austria
- Bernhard Paulweber
- Department of Internal Medicine, St. Johanns University Hospital, Paracelsus Medical University, Salzburg, Austria
- Eugen Trinka
- Department of Neurology, Christian Doppler Clinic, Paracelsus Medical University, Salzburg, Austria
- Eugen Trinka
- Department of Public Health, Health Services Research and Health Technology Assessment, UMIT-University for Health Sciences, Medical Informatics and Technology, Hall in Tirol, Austria
- Eugen Trinka
- Neuroscience Institute, Christian Doppler University Hospital, Paracelsus Medical University and Centre for Cognitive Neuroscience Salzburg, Salzburg, Austria
- Janne Cadamuro
- 0Department of Laboratory Medicine, University Hospital SALK, Salzburg, Austria
- Martin Drerup
- 1Department of Urology, Paracelsus Medical University, Salzburg, Austria
- Katharina Schallmoser
- Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg, Austria
- Katharina Schallmoser
- 2Department of Transfusion Medicine, Paracelsus Medical University, Salzburg, Austria
- Ludwig Aigner
- Institute of Molecular Regenerative Medicine, Paracelsus Medical University, Salzburg, Austria
- Ludwig Aigner
- Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg, Austria
- Ludwig Aigner
- 3Austrian Cluster for Tissue Regeneration, Vienna, Austria
- Kathrin M. Kniewallner
- Institute of Molecular Regenerative Medicine, Paracelsus Medical University, Salzburg, Austria
- Kathrin M. Kniewallner
- Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University, Salzburg, Austria
- DOI
- https://doi.org/10.3389/fmolb.2023.1196083
- Journal volume & issue
-
Vol. 10
Abstract
Introduction: Alzheimer’s disease (AD) and aging are associated with platelet hyperactivity. However, the mechanisms underlying abnormal platelet function in AD and aging are yet poorly understood.Methods: To explore the molecular profile of AD and aged platelets, we investigated platelet activation (i.e., CD62P expression), proteome and transcriptome in AD patients, non-demented elderly, and young individuals as controls.Results: AD, aged and young individuals showed similar levels of platelet activation based on CD62P expression. However, AD and aged individuals had a proteomic signature suggestive of increased platelet activation compared with young controls. Transcriptomic profiling suggested the dysregulation of proteolytic machinery involved in regulating platelet function, particularly the ubiquitin-proteasome system in AD and autophagy in aging. The functional implication of these transcriptomic alterations remains unclear and requires further investigation.Discussion: Our data strengthen the evidence of enhanced platelet activation in aging and provide a first glimpse of the platelet transcriptomic changes occurring in AD.
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