FAT/CD36 expression is not ablated in spontaneously hypertensive rats

Arend Bonen; Xiao-Xia Han; Narendra N. Tandon; Jan F.C. Glatz; James Lally; Laelie A. Snook; Joost J. F.P. Luiken

Journal of Lipid Research (Apr 2009)

FAT/CD36 expression is not ablated in spontaneously hypertensive rats

Arend Bonen,
Xiao-Xia Han,
Narendra N. Tandon,
Jan F.C. Glatz,
James Lally,
Laelie A. Snook,
Joost J. F.P. Luiken

Affiliations

Arend Bonen: Department of Human Health and Nutritional Sciences University of -Guelph, Guelph, Ontario, N1G 2W1, Canada; Thrombosis Research Laboratory, Otsuka Maryland Medicinal Laboratories 9900 Medical Center Drive, Rockville, MD 20850, Maastricht University, 6200-MD Maastricht, The Netherlands; Department of Molecular Genetics, Maastricht University, 6200-MD Maastricht, The Netherlands
Xiao-Xia Han: Department of Human Health and Nutritional Sciences University of -Guelph, Guelph, Ontario, N1G 2W1, Canada; Thrombosis Research Laboratory, Otsuka Maryland Medicinal Laboratories 9900 Medical Center Drive, Rockville, MD 20850, Maastricht University, 6200-MD Maastricht, The Netherlands; Department of Molecular Genetics, Maastricht University, 6200-MD Maastricht, The Netherlands
Narendra N. Tandon: Department of Human Health and Nutritional Sciences University of -Guelph, Guelph, Ontario, N1G 2W1, Canada; Thrombosis Research Laboratory, Otsuka Maryland Medicinal Laboratories 9900 Medical Center Drive, Rockville, MD 20850, Maastricht University, 6200-MD Maastricht, The Netherlands; Department of Molecular Genetics, Maastricht University, 6200-MD Maastricht, The Netherlands
Jan F.C. Glatz: Department of Human Health and Nutritional Sciences University of -Guelph, Guelph, Ontario, N1G 2W1, Canada; Thrombosis Research Laboratory, Otsuka Maryland Medicinal Laboratories 9900 Medical Center Drive, Rockville, MD 20850, Maastricht University, 6200-MD Maastricht, The Netherlands; Department of Molecular Genetics, Maastricht University, 6200-MD Maastricht, The Netherlands
James Lally: Department of Human Health and Nutritional Sciences University of -Guelph, Guelph, Ontario, N1G 2W1, Canada; Thrombosis Research Laboratory, Otsuka Maryland Medicinal Laboratories 9900 Medical Center Drive, Rockville, MD 20850, Maastricht University, 6200-MD Maastricht, The Netherlands; Department of Molecular Genetics, Maastricht University, 6200-MD Maastricht, The Netherlands
Laelie A. Snook: Department of Human Health and Nutritional Sciences University of -Guelph, Guelph, Ontario, N1G 2W1, Canada; Thrombosis Research Laboratory, Otsuka Maryland Medicinal Laboratories 9900 Medical Center Drive, Rockville, MD 20850, Maastricht University, 6200-MD Maastricht, The Netherlands; Department of Molecular Genetics, Maastricht University, 6200-MD Maastricht, The Netherlands
Joost J. F.P. Luiken: Department of Human Health and Nutritional Sciences University of -Guelph, Guelph, Ontario, N1G 2W1, Canada; Thrombosis Research Laboratory, Otsuka Maryland Medicinal Laboratories 9900 Medical Center Drive, Rockville, MD 20850, Maastricht University, 6200-MD Maastricht, The Netherlands; Department of Molecular Genetics, Maastricht University, 6200-MD Maastricht, The Netherlands

Journal volume & issue: Vol. 50, no. 4
pp. 740 – 748

Abstract

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There is doubt whether spontaneously hypertensive rats (SHR; North American strain) are null for fatty acid translocase (FAT/CD36). Therefore, we examined whether FAT/CD36 is expressed in heart, muscle, liver and adipose tissue in SHR. Insulin resistance was present in SHR skeletal muscle. We confirmed that SHR expressed aberrant FAT mRNAs in key metabolic tissues; namely, the major 2.9 kb transcript was not expressed, but 3.8 and 5.4 kb transcripts were present. Despite this, FAT/CD36 protein was expressed in all tissues, although there were tissue-specific reductions in FAT/CD36 protein expression and plasmalemmal content, ranging from 26–85%. Fatty acid transport was reduced in adipose tissue (−50%) and was increased in liver (+47%). Normal rates of fatty acid transport occurred in heart and muscle, possibly due to compensatory upregulation of plasmalemmal fatty acid binding protein (FABPpm) in red (+123%) and white muscle (+110%). In conclusion, SHRs (North American strain) are not a natural FAT/CD36 null model, the North American strain of SHR express FAT/CD36, albeit at reduced levels.

Published in Journal of Lipid Research

ISSN: 0022-2275 (Print); 1539-7262 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: https://www.journals.elsevier.com/journal-of-lipid-research

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