Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea

Victor R. C. Merino; Viviane Nakano; Sydney M. Finegold; Mario J. Avila-Campos

doi:10.1155/2014/594014

Scientifica (Jan 2014)

Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea

Victor R. C. Merino,
Viviane Nakano,
Sydney M. Finegold,
Mario J. Avila-Campos

Affiliations

Victor R. C. Merino: Anaerobe Laboratory, Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, Brazil
Viviane Nakano: Anaerobe Laboratory, Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, Brazil
Sydney M. Finegold: Veterans Affairs, West Los Angeles Medical Center, Los Angeles, CA, USA
Mario J. Avila-Campos: Anaerobe Laboratory, Department of Microbiology, Institute of Biomedical Science, University of São Paulo, São Paulo, SP, Brazil

DOI: https://doi.org/10.1155/2014/594014
Journal volume & issue: Vol. 2014

Abstract

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The presence of gene 16S rRNA and genes encoding toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtA/cdtB) of Clostridium difficile in stool samples from children with (110) and without (150) diarrhea was determined by using a TaqMan system. Fifty-seven (21.9%) out of 260 stool samples harbored the 16S rRNA gene. The genetic profile of tcdA+/tcdB− and cdtA+/cdtB+ was verified in one C. difficile-positive diarrhea sample and of tcdA+/tcdB+ in three C. difficile-positive nondiarrhea samples. The presence of tcdA+/tcdB+ in stools obtained from children without diarrhea, suggests that they were asymptomatic carriers of toxigenic strains.

Published in Scientifica

ISSN: 2090-908X (Online)
Publisher: Wiley
Country of publisher: United Kingdom
LCC subjects: Medicine; Science
Website: https://onlinelibrary.wiley.com/journal/6168

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