Two metallothionein gene family members in buckwheat: Expression analysis in flooding stress using Real Time RT-PCR technology

Abstract

Metallothioneins (MTs) are an extensive and diverse family of small cysteine-rich proteins with metal-binding ability that are involved in metal homeostasis and detoxification. Two cDNA clones of the MT3 type, differing in 3’ UTRs, were isolated from the developing buckwheat seed cDNA library. Following sequence analyses, expression profiles during flooding stress were monitored by Real Time RT PCR technology.

Keywords

• buckwheat
• flooding
• metallothionein
• Real Time RT-PCR