A biosensor-based framework to measure latent proteostasis capacity

Rebecca J. Wood; Angelique R. Ormsby; Mona Radwan; Dezerae Cox; Abhishek Sharma; Tobias Vöpel; Simon Ebbinghaus; Mikael Oliveberg; Gavin E. Reid; Alex Dickson; Danny M. Hatters

doi:10.1038/s41467-017-02562-5

Nature Communications (Jan 2018)

A biosensor-based framework to measure latent proteostasis capacity

Rebecca J. Wood,
Angelique R. Ormsby,
Mona Radwan,
Dezerae Cox,
Abhishek Sharma,
Tobias Vöpel,
Simon Ebbinghaus,
Mikael Oliveberg,
Gavin E. Reid,
Alex Dickson,
Danny M. Hatters

Affiliations

Rebecca J. Wood: Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne
Angelique R. Ormsby: Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne
Mona Radwan: Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne
Dezerae Cox: Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne
Abhishek Sharma: Department of Physical Chemistry II, Ruhr-University Bochum
Tobias Vöpel: Department of Physical Chemistry II, Ruhr-University Bochum
Simon Ebbinghaus: Department of Physical Chemistry II, Ruhr-University Bochum
Mikael Oliveberg: Department of Biochemistry and Biophysics, Arrhenius Laboratories of Natural Sciences, Stockholm University
Gavin E. Reid: Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne
Alex Dickson: Department of Biochemistry & Molecular Biology, Michigan State University
Danny M. Hatters: Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne

DOI: https://doi.org/10.1038/s41467-017-02562-5
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 10

Abstract

Read online

A pool of quality control proteins (QC) maintains the protein-folding homeostasis in the cell, but its quantitative analysis is challenging. Here the authors develop a FRET sensor based on the protein barnase, able to quantify QC holdase activity and its ability to suppress protein aggregation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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