Single-Atomic Site Catalyst Enhanced Lateral Flow Immunoassay for Point-of-Care Detection of Herbicide

Zhaoyuan Lyu; Shichao Ding; Peter Tieu; Lingzhe Fang; Xin Li; Tao Li; Xiaoqing Pan; Mark H. Engelhard; Xiaofan Ruan; Dan Du; Suiqiong Li; Yuehe Lin

doi:10.34133/2022/9823290

Research (Jan 2022)

Single-Atomic Site Catalyst Enhanced Lateral Flow Immunoassay for Point-of-Care Detection of Herbicide

Zhaoyuan Lyu,
Shichao Ding,
Peter Tieu,
Lingzhe Fang,
Xin Li,
Tao Li,
Xiaoqing Pan,
Mark H. Engelhard,
Xiaofan Ruan,
Dan Du,
Suiqiong Li,
Yuehe Lin

Affiliations

Zhaoyuan Lyu: School of Mechanical and Material Engineering, Washington State University, Pullman, WA 99164, USA
Shichao Ding: School of Mechanical and Material Engineering, Washington State University, Pullman, WA 99164, USA
Peter Tieu: Department of Chemistry, University of California, Irvine, Irvine, CA 92697, USA
Lingzhe Fang: Department of Chemistry and Biochemistry, Northern Illinois University, DeKalb, IL 60115, USA
Xin Li: School of Mechanical and Material Engineering, Washington State University, Pullman, WA 99164, USA
Tao Li: Department of Chemistry and Biochemistry, Northern Illinois University, DeKalb, IL 60115, USA; X-ray Science Division, Argonne National Laboratory, Lemont, IL 60439, USA
Xiaoqing Pan: Irvine Materials Research Institute (IMRI), Department of Materials Science and Engineering, University of California, Irvine, Irvine, CA 92697, USA
Mark H. Engelhard: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99354, USA
Xiaofan Ruan: School of Mechanical and Material Engineering, Washington State University, Pullman, WA 99164, USA
Dan Du: School of Mechanical and Material Engineering, Washington State University, Pullman, WA 99164, USA
Suiqiong Li: DL ADV-Tech, Pullman, WA 99163, USA
Yuehe Lin: School of Mechanical and Material Engineering, Washington State University, Pullman, WA 99164, USA

DOI: https://doi.org/10.34133/2022/9823290
Journal volume & issue: Vol. 2022

Abstract

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Point-of-care (POC) detection of herbicides is of great importance due to their impact on the environment and potential risks to human health. Here, we design a single-atomic site catalyst (SASC) with excellent peroxidase-like (POD-like) catalytic activity, which enhances the detection performance of corresponding lateral flow immunoassay (LFIA). The iron single-atomic site catalyst (Fe-SASC) is synthesized from hemin-doped ZIF-8, creating active sites that mimic the Fe active center coordination environment of natural enzyme and their functions. Due to its atomically dispersed iron active sites that result in maximum utilization of active metal atoms, the Fe-SASC exhibits superior POD-like activity, which has great potential to replace its natural counterparts. Also, the catalytic mechanism of Fe-SASC is systematically investigated. Utilizing its outstanding catalytic activity, the Fe-SASC is used as label to construct LFIA (Fe-SASC-LFIA) for herbicide detection. The 2,4-dichlorophenoxyacetic acid (2,4-D) is selected as a target here, since it is a commonly used herbicide as well as a biomarker for herbicide exposure evaluation. A linear detection range of 1-250 ng/mL with a low limit of detection (LOD) of 0.82 ng/mL has been achieved. Meanwhile, excellent specificity and selectivity towards 2,4-D have been obtained. The outstanding detection performance of the Fe-SASC-LFIA has also been demonstrated in the detection of human urine samples, indicating the practicability of this POC detection platform for analyzing the 2,4-D exposure level of a person. We believe this proposed Fe-SASC-LFIA has potential as a portable, rapid, and high-sensitive POC detection strategy for pesticide exposure evaluation.

Published in Research

ISSN: 2096-5168 (Print); 2639-5274 (Online)
Publisher: American Association for the Advancement of Science (AAAS)
Country of publisher: United States
LCC subjects: Science
Website: https://spj.science.org/journal/research

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