Food and Agricultural Immunology (Jan 2018)

Immunochromatographic strip for rapid detection of phenylethanolamine A

  • Wei Jiang,
  • Lu Zeng,
  • Liqiang Liu,
  • Shanshan Song,
  • Hua Kuang

DOI
https://doi.org/10.1080/09540105.2017.1364709
Journal volume & issue
Vol. 29, no. 1
pp. 182 – 192

Abstract

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A monoclonal antibody (mAb) directed against phenylethanolamine A (PEA) was successfully prepared with the immunization of mice and cell fusion, and used to develop a detection method for a colloidal gold immunoassay. The 50% inhibitory concentration of mAb PEA-2G10 was 0.213 ng/mL, the limit of detection was 0.033 ng/mL, and its detection range 0.033–0.679 ng/mL. The recovery rate for PEA in swine urine was 84.5–90.8%, with good stability and accuracy. “Dry” immunoassay test strips were successfully prepared based on antibody 2G10. When the strip was tested in swine urine, the cutoff value for PEA was 5 ng/mL, with highly sensitivity. The method developed here can be used to test swine urine on-site for the rapid detection of PEA.

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