The dopamine transporter antiports potassium to increase the uptake of dopamine

Solveig G. Schmidt; Mette Galsgaard Malle; Anne Kathrine Nielsen; Søren S.-R. Bohr; Ciara F. Pugh; Jeppe C. Nielsen; Ida H. Poulsen; Kasper D. Rand; Nikos S. Hatzakis; Claus J. Loland

doi:10.1038/s41467-022-30154-5

Nature Communications (May 2022)

The dopamine transporter antiports potassium to increase the uptake of dopamine

Solveig G. Schmidt,
Mette Galsgaard Malle,
Anne Kathrine Nielsen,
Søren S.-R. Bohr,
Ciara F. Pugh,
Jeppe C. Nielsen,
Ida H. Poulsen,
Kasper D. Rand,
Nikos S. Hatzakis,
Claus J. Loland

Affiliations

Solveig G. Schmidt: Laboratory for Membrane Protein Dynamics, Department of Neuroscience, Faculty of Health and Medical Sciences, University of Copenhagen
Mette Galsgaard Malle: Nano-Science Center, Department of Chemistry, Faculty of Science, University of Copenhagen
Anne Kathrine Nielsen: Laboratory for Membrane Protein Dynamics, Department of Neuroscience, Faculty of Health and Medical Sciences, University of Copenhagen
Søren S.-R. Bohr: Nano-Science Center, Department of Chemistry, Faculty of Science, University of Copenhagen
Ciara F. Pugh: Laboratory for Membrane Protein Dynamics, Department of Neuroscience, Faculty of Health and Medical Sciences, University of Copenhagen
Jeppe C. Nielsen: Laboratory for Membrane Protein Dynamics, Department of Neuroscience, Faculty of Health and Medical Sciences, University of Copenhagen
Ida H. Poulsen: Laboratory for Membrane Protein Dynamics, Department of Neuroscience, Faculty of Health and Medical Sciences, University of Copenhagen
Kasper D. Rand: Protein Analysis Group, Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen
Nikos S. Hatzakis: Nano-Science Center, Department of Chemistry, Faculty of Science, University of Copenhagen
Claus J. Loland: Laboratory for Membrane Protein Dynamics, Department of Neuroscience, Faculty of Health and Medical Sciences, University of Copenhagen

DOI: https://doi.org/10.1038/s41467-022-30154-5
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 12

Abstract

Read online

The dopamine transporter, DAT, controls dopamine signaling by facilitating its reuptake using the Na+ gradient as driving force. Here, the authors uncover that an antiport of K+ ions also contributes to setting the rate of DAT-mediated dopamine clearance.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal