An improved method for murine laser-induced choroidal neovascularization lesion quantification from optical coherence tomography images
Nathan R. Jensen,
Nathan Lambert-Cheatham,
Gabriella D. Hartman,
Anbukkarasi Muniyandi,
Bomina Park,
Kamakshi Sishtla,
Timothy W. Corson
Affiliations
Nathan R. Jensen
Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, United States; The University of Utah School of Medicine, 30N 1900 E, Salt Lake City, UT, United States
Nathan Lambert-Cheatham
Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, United States
Gabriella D. Hartman
Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, United States; Stark Neurosciences Research Institute, Indiana University School of Medicine, United States
Anbukkarasi Muniyandi
Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, United States
Bomina Park
Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, United States; Department of Pharmacology and Toxicology, Indiana University School of Medicine, United States
Kamakshi Sishtla
Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, United States
Timothy W. Corson
Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, United States; Stark Neurosciences Research Institute, Indiana University School of Medicine, United States; Department of Pharmacology and Toxicology, Indiana University School of Medicine, United States; Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 1160 West Michigan Street, Indianapolis, IN 46202, United States; Corresponding author at: Department of Ophthalmology, Indiana University School of Medicine, 1160 West Michigan Street, Indianapolis, IN 46202, United States.
Laser-induced choroidal neovascularization (L-CNV) in murine models is a standard method for assessing therapies, genetics, and mechanisms relevant to the blinding eye disease neovascular or “wet” age-related macular degeneration. The ex vivo evaluation of these lesions involves confocal microscopy analysis. In vivo evaluation via optical coherence tomography (OCT) has previously been established and allows longitudinal assessment of lesion development. However, to produce robust data, evaluation of many lesions may be required, which can be a slow, arduous process. A prior, manual method for quantifying these lesions as ellipsoids from orthogonal OCT images was effective but time consuming. We therefore developed an OCT lesion quantification that is simplified, streamlined, and less time-consuming.
