Scientific Reports (Jul 2017)

Suppressed autophagic response underlies augmentation of renal ischemia/reperfusion injury by type 2 diabetes

  • Shingo Muratsubaki,
  • Atsushi Kuno,
  • Masaya Tanno,
  • Takayuki Miki,
  • Toshiyuki Yano,
  • Hirohito Sugawara,
  • Satoru Shibata,
  • Koki Abe,
  • Satoko Ishikawa,
  • Kouhei Ohno,
  • Yukishige Kimura,
  • Yuki Tatekoshi,
  • Kei Nakata,
  • Wataru Ohwada,
  • Masashi Mizuno,
  • Tetsuji Miura

DOI
https://doi.org/10.1038/s41598-017-05667-5
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 13

Abstract

Read online

Abstract Diabetes mellitus is a major risk factor for acute kidney injury (AKI). Here, we hypothesized that suppression of autophagic response underlies aggravation of renal ischemia/reperfusion (I/R) injury by type 2 diabetes mellitus (T2DM). In OLETF, a rat model of T2DM, and its non-diabetic control, LETO, AKI was induced by unilateral nephrectomy and 30-min occlusion and 24-h reperfusion of the renal artery in the contralateral kidney. Levels of serum creatinine and blood urea nitrogen and tubular injury score after I/R were significantly higher in OLETF than in LETO. Administration of chloroquine, a widely used autophagy inhibitor, aggravated I/R-induced renal injury in LETO, but not in OLETF. In contrast to LETO, OLETF exhibited no increase in autophagosomes in the proximal tubules after I/R. Immunoblotting showed that I/R activated the AMPK/ULK1 pathway in LETO but not in OLETF, and mTORC1 activation after I/R was enhanced in OLETF. Treatment of OLETF with rapamycin, an mTORC1 inhibitor, partially restored autophagic activation in response to I/R and significantly attenuated I/R-induced renal injury. Collectively, these findings indicate that suppressed autophagic activation in proximal tubules by impaired AMPK/ULK1 signaling and upregulated mTORC1 activation underlies T2DM-induced worsening of renal I/R injury.