Generation of induced pluripotent stem cell line, NIMHi009-A, from PBMCs of an adult healthy male

Gautham Arunachal; Madhura Milind Nimonkar; Kenchaiah Raghavendra; Ghati K Chetan; Bhupesh Mehta; Yogananda S. Markandeya

Stem Cell Research (Apr 2024)

Generation of induced pluripotent stem cell line, NIMHi009-A, from PBMCs of an adult healthy male

Gautham Arunachal,
Madhura Milind Nimonkar,
Kenchaiah Raghavendra,
Ghati K Chetan,
Bhupesh Mehta,
Yogananda S. Markandeya

Affiliations

Gautham Arunachal: Department of Human Genetics, National Institute of Mental Health and Neuro Sciences (NIMHANS), Bengaluru, Karnataka, India
Madhura Milind Nimonkar: Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India
Kenchaiah Raghavendra: Department of Neurology, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India
Ghati K Chetan: Department of Human Genetics, National Institute of Mental Health and Neuro Sciences (NIMHANS), Bengaluru, Karnataka, India
Bhupesh Mehta: Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India
Yogananda S. Markandeya: Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India; Corresponding author.

Journal volume & issue: Vol. 76
p. 103349

Abstract

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Human induced pluripotent stem cells provide an exceptional platform for studying pathogenesis in vitro. We, therefore, have generated and characterized human induced pluripotent stem cell (iPSC) line NIMHi009-A derived from peripheral blood mononuclear cells (PBMCs) of healthy adult male control for an epileptic patient carrying voltage gated sodium channel mutation, using Sendai virus-based reprogramming. The generated iPSCs express pluripotency genes and can spontaneously differentiate into three germ layers. These cells display a normal karyotype and are free of mycoplasma. The iPSC line NIMHi009-A can be used as healthy control for modelling various diseases and screening for drugs.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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