Insights into the molecular basis of gastric mucosa as a first step for using Raman microscopy in paediatrics
Jiří Bufka,
Lenka Vaňková,
Josef Sýkora,
Magdaléna Daumová,
Petr Bouř,
Jan Schwarz
Affiliations
Jiří Bufka
Department of Paediatrics, Faculty of Medicine in Pilsen, Faculty Hospital, Charles University in Prague, Pilsen, Czech Republic; Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences Prague, Czech Republic; Corresponding author. Department of Paediatrics, Faculty of Medicine in Pilsen, Faculty Hospital, Charles University in Prague, Pilsen, Czech Republic, Alej Svobody 80, Pilsen, 323 00, Czech Republic.
Lenka Vaňková
Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University, Czech Republic
Josef Sýkora
Department of Paediatrics, Faculty of Medicine in Pilsen, Faculty Hospital, Charles University in Prague, Pilsen, Czech Republic
Magdaléna Daumová
Sikl's Department of Pathology, Faculty of Medicine in Pilsen, Charles University, Czech Republic
Petr Bouř
Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences Prague, Czech Republic; Corresponding author.
Jan Schwarz
Department of Paediatrics, Faculty of Medicine in Pilsen, Faculty Hospital, Charles University in Prague, Pilsen, Czech Republic
Recent advances in endoscopic technology have allowed detailed observation of the gastric mucosa, including Raman microscopy and spectroscopy. To explore the possibilities for future diagnostic use, we discuss the measurements and molecular markers found in this tissue. The Raman spectra of 16 samples of antral mucosa and 16 samples of corpus gastric mucosa obtained from healthy donors were analysed. A stable protocol for measuring reproducible spectra was established. These data suggest that many biomarkers can be used for the rapid analysis of metabolic states and future investigations into the pathogenesis of gastrointestinal diseases.
