The Effect of Eight Weeks of Endurance Training on amount of KIF5B Protein in Soleus and Extensor Digital Longus Muscle Tissue in Aged Male Wistar Race Rats

Abstract

Background: The aim of the present study was determination of the effect of eight weeks of Endurance Training (ET) on amount of KIF5B protein in Soleus and Extensor Digital Longus muscle tissue in aged male Wistar race rats. Methods: 12 Aged male rats with an average age of eighteen to twenty-four months and an average weight of 407.83 ± 21.29 grams and 6 young rats with an average age of eight to ten weeks and an average weight of 180.67 ± 10.36 grams were randomly divided into 3 groups of six series (aged endurance training, aged sham exercise and young sham exercise). The aged endurance training group ran continuously on a treadmill for 15 to 60 minutes with intensity of 7.5-15 meter/ Min, 5 days a week for eight weeks. To measure the amount of KIF5B protein, ELISA method and to test the hypotheses, one-way analysis of variance and Tukey's post hoc test were used. Findings: Following aging, the amount of KIF5B protein decreased significantly in soleus and EDL muscle. Eight weeks of ET caused a significant increase in the amount of KIF5B protein in the soleus and EDL compared to the aged sham exercise. The ratio of the weight of Soleus and EDL to body weight in the ET group had a significant increase compared to the aged sham exercise. Conclusion: Following a regular training period of ET, the amount of KIF5B protein in the soleus and EDL muscles as well as the peak running speed of aged male rats increased and reduced sarcopenia by increasing the relative weight of soleus and EDL muscles in them.

Keywords

• exercise
• sarcopenia
• skeletal muscle
• aging