A New Diterpenoid of Indonesian <i>Scoparia dulcis</i> Linn: Isolation and Cytotoxic Activity against MCF-7 and T47D Cell Lines

Abstract

Scoparia dulcis Linn plays an important role in treatment because it contains active compounds that are proven to have a variety of activities, including cytotoxicity on various cancer cells. The objective of this study is to isolate and identify the cytotoxic compounds in the ethyl acetate fraction of Scoparia dulcis, observe cell cycle inhibition and induction of apoptosis in vitro, and carry out molecular studies using in silico studies. A new diterpene compound was isolated from the ethyl acetate fraction of Scoparia dulcis L. of Indonesian origin. Chromatographic methods were used to isolate the compound, spectroscopic methods were used to elucidate its structure, and these data were compared with those reported in the literature. The compound was tested for its cytotoxic activity against two breast cancer cells (MCF-7 and T47D). The results of the isolated compound showed a cytotoxic effect on MCF-7 and T47D breast cancer cells at IC50 70.56 ± 1.54 and S. dulcis demonstrated anticancer activity based on in vitro and in silico studies.

Keywords

• <i>Scoparia dulcis</i>
• 2α-hydroxyscopadiol
• cytotoxic
• MCF-7
• T47D
• molecular docking