Drug-Releasing Antibacterial Coating Made from Nano-Hydroxyapatite Using the Sonocoating Method
Khaled AbouAitah,
Monika Bil,
Elzbieta Pietrzykowska,
Urszula Szałaj,
Damian Fudala,
Bartosz Woźniak,
Justyna Nasiłowska,
Anna Swiderska-Sroda,
Maciej Lojkowski,
Barbara Sokołowska,
Wojciech Swieszkowski,
Witold Lojkowski
Affiliations
Khaled AbouAitah
Laboratory of Nanostructures and Nanomedicine, Institute of High Pressure Physics, Polish Academy of Sciences, 29/37 Sokolowska Street, 01142 Warsaw, Poland
Monika Bil
Centre for Advanced Materials and Technologies, Warsaw University of Technology, Poleczki 19, 02822 Warsaw, Poland
Elzbieta Pietrzykowska
Laboratory of Nanostructures and Nanomedicine, Institute of High Pressure Physics, Polish Academy of Sciences, 29/37 Sokolowska Street, 01142 Warsaw, Poland
Urszula Szałaj
Laboratory of Nanostructures and Nanomedicine, Institute of High Pressure Physics, Polish Academy of Sciences, 29/37 Sokolowska Street, 01142 Warsaw, Poland
Damian Fudala
Laboratory of Nanostructures and Nanomedicine, Institute of High Pressure Physics, Polish Academy of Sciences, 29/37 Sokolowska Street, 01142 Warsaw, Poland
Bartosz Woźniak
Laboratory of Nanostructures and Nanomedicine, Institute of High Pressure Physics, Polish Academy of Sciences, 29/37 Sokolowska Street, 01142 Warsaw, Poland
Justyna Nasiłowska
Department of Microbiology, Prof. Wacław Dąbrowski Institute of Agriculture and Food Biotechnology–State Research Institute, 36 Rakowiecka Street, 02532 Warsaw, Poland
Anna Swiderska-Sroda
Laboratory of Nanostructures and Nanomedicine, Institute of High Pressure Physics, Polish Academy of Sciences, 29/37 Sokolowska Street, 01142 Warsaw, Poland
Maciej Lojkowski
Faculty of Materials Science and Engineering, Warsaw University of Technology, 141 Woloska Street, 02507 Warsaw, Poland
Barbara Sokołowska
Department of Microbiology, Prof. Wacław Dąbrowski Institute of Agriculture and Food Biotechnology–State Research Institute, 36 Rakowiecka Street, 02532 Warsaw, Poland
Wojciech Swieszkowski
Faculty of Materials Science and Engineering, Warsaw University of Technology, 141 Woloska Street, 02507 Warsaw, Poland
Witold Lojkowski
Laboratory of Nanostructures and Nanomedicine, Institute of High Pressure Physics, Polish Academy of Sciences, 29/37 Sokolowska Street, 01142 Warsaw, Poland
Medical implant use is associated with a risk of infection caused by bacteria on their surface. Implants with a surface that has both bone growth-promoting properties and antibacterial properties are of interest in orthopedics. In the current study, we fabricated a bioactive coating of hydroxyapatite nanoparticles on polyether ether ketone (PEEK) using the sonocoating method. The sonocoating method creates a layer by immersing the object in a suspension of nanoparticles in water and applying a high-power ultrasound. We show that the simple layer fabrication method results in a well-adhering layer with a thickness of 219 nm to 764 nm. Dropping cefuroxime sodium salt (Cef) antibiotic on the coated substrate creates a layer with a drug release effect and antibacterial activity against Staphylococcus aureus. We achieved a concentration of up to 1 mg of drug per cm2 of the coated substrate. In drug release tests, an initial burst was observed within 24 h, accompanied by a linear stable release effect. The drug-loaded implants exhibited sufficient activity against S. aureus for 24 and 168 h. Thus, the simple method we present here produces a biocompatible coating that can be soaked with antibiotics for antibacterial properties and can be used for a range of medical implants.
