Frontiers in Genetics (Jun 2022)
Transcriptome Analysis Reveals that Exogenous Melatonin Confers Lilium Disease Resistance to Botrytis elliptica
- Xuehua Xie,
- Xuehua Xie,
- Xuehua Xie,
- Xuehua Xie,
- Xuehua Xie,
- Xuehua Xie,
- Yu Han,
- Yu Han,
- Yu Han,
- Yu Han,
- Yu Han,
- Yu Han,
- Xi Yuan,
- Xi Yuan,
- Xi Yuan,
- Xi Yuan,
- Xi Yuan,
- Xi Yuan,
- Man Zhang,
- Man Zhang,
- Man Zhang,
- Man Zhang,
- Man Zhang,
- Man Zhang,
- Ping Li,
- Ping Li,
- Ping Li,
- Ping Li,
- Ping Li,
- Ping Li,
- Aiqin Ding,
- Aiqin Ding,
- Aiqin Ding,
- Aiqin Ding,
- Aiqin Ding,
- Aiqin Ding,
- Jia Wang,
- Jia Wang,
- Jia Wang,
- Jia Wang,
- Jia Wang,
- Jia Wang,
- Tangren Cheng,
- Tangren Cheng,
- Tangren Cheng,
- Tangren Cheng,
- Tangren Cheng,
- Tangren Cheng,
- Qixiang Zhang,
- Qixiang Zhang,
- Qixiang Zhang,
- Qixiang Zhang,
- Qixiang Zhang,
- Qixiang Zhang
Affiliations
- Xuehua Xie
- Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Forestry University, Beijing, China
- Xuehua Xie
- National Engineering Research Center for Floriculture, Beijing Forestry University, Beijing, China
- Xuehua Xie
- Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing, China
- Xuehua Xie
- Engineering Research Center of Landscape Environment of Ministry of Education, Beijing Forestry University, Beijing, China
- Xuehua Xie
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, Beijing, China
- Xuehua Xie
- School of Landscape Architecture, Beijing Forestry University, Beijing, China
- Yu Han
- Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Forestry University, Beijing, China
- Yu Han
- National Engineering Research Center for Floriculture, Beijing Forestry University, Beijing, China
- Yu Han
- Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing, China
- Yu Han
- Engineering Research Center of Landscape Environment of Ministry of Education, Beijing Forestry University, Beijing, China
- Yu Han
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, Beijing, China
- Yu Han
- School of Landscape Architecture, Beijing Forestry University, Beijing, China
- Xi Yuan
- Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Forestry University, Beijing, China
- Xi Yuan
- National Engineering Research Center for Floriculture, Beijing Forestry University, Beijing, China
- Xi Yuan
- Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing, China
- Xi Yuan
- Engineering Research Center of Landscape Environment of Ministry of Education, Beijing Forestry University, Beijing, China
- Xi Yuan
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, Beijing, China
- Xi Yuan
- School of Landscape Architecture, Beijing Forestry University, Beijing, China
- Man Zhang
- Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Forestry University, Beijing, China
- Man Zhang
- National Engineering Research Center for Floriculture, Beijing Forestry University, Beijing, China
- Man Zhang
- Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing, China
- Man Zhang
- Engineering Research Center of Landscape Environment of Ministry of Education, Beijing Forestry University, Beijing, China
- Man Zhang
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, Beijing, China
- Man Zhang
- School of Landscape Architecture, Beijing Forestry University, Beijing, China
- Ping Li
- Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Forestry University, Beijing, China
- Ping Li
- National Engineering Research Center for Floriculture, Beijing Forestry University, Beijing, China
- Ping Li
- Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing, China
- Ping Li
- Engineering Research Center of Landscape Environment of Ministry of Education, Beijing Forestry University, Beijing, China
- Ping Li
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, Beijing, China
- Ping Li
- School of Landscape Architecture, Beijing Forestry University, Beijing, China
- Aiqin Ding
- Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Forestry University, Beijing, China
- Aiqin Ding
- National Engineering Research Center for Floriculture, Beijing Forestry University, Beijing, China
- Aiqin Ding
- Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing, China
- Aiqin Ding
- Engineering Research Center of Landscape Environment of Ministry of Education, Beijing Forestry University, Beijing, China
- Aiqin Ding
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, Beijing, China
- Aiqin Ding
- School of Landscape Architecture, Beijing Forestry University, Beijing, China
- Jia Wang
- Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Forestry University, Beijing, China
- Jia Wang
- National Engineering Research Center for Floriculture, Beijing Forestry University, Beijing, China
- Jia Wang
- Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing, China
- Jia Wang
- Engineering Research Center of Landscape Environment of Ministry of Education, Beijing Forestry University, Beijing, China
- Jia Wang
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, Beijing, China
- Jia Wang
- School of Landscape Architecture, Beijing Forestry University, Beijing, China
- Tangren Cheng
- Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Forestry University, Beijing, China
- Tangren Cheng
- National Engineering Research Center for Floriculture, Beijing Forestry University, Beijing, China
- Tangren Cheng
- Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing, China
- Tangren Cheng
- Engineering Research Center of Landscape Environment of Ministry of Education, Beijing Forestry University, Beijing, China
- Tangren Cheng
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, Beijing, China
- Tangren Cheng
- School of Landscape Architecture, Beijing Forestry University, Beijing, China
- Qixiang Zhang
- Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Forestry University, Beijing, China
- Qixiang Zhang
- National Engineering Research Center for Floriculture, Beijing Forestry University, Beijing, China
- Qixiang Zhang
- Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing, China
- Qixiang Zhang
- Engineering Research Center of Landscape Environment of Ministry of Education, Beijing Forestry University, Beijing, China
- Qixiang Zhang
- Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, Beijing, China
- Qixiang Zhang
- School of Landscape Architecture, Beijing Forestry University, Beijing, China
- DOI
- https://doi.org/10.3389/fgene.2022.892674
- Journal volume & issue
-
Vol. 13
Abstract
Leaf blight, caused by Botrytis elliptica (Berk.) Cooke, is a devastating disease that limits the production of Lilium in China and in other countries worldwide. Numerous studies have indicated that plants have evolved sophisticated and effective signal transduction and defense-related pathways in response to pathogen invasion. Recently, particular attention has been given to the action(s) of melatonin in plants in response to biotic stress, and the role of melatonin in plant–pathogen interactions has also been discussed. In this study, RNA-seq was applied to analyze the transcriptomic changes in Lilium leaves that were pre-treated and post-treated with melatonin after B. elliptica infection for 0, 12, 24, 36, and 72 h and then compare those changes with those of the control. Treatment with exogenous melatonin and infection with B. elliptica caused differential expression of a large number of genes in Lilium leaves. KEGG pathway analysis showed that, after melatonin treatment, the defense-related DEGs were mainly enriched in plant–pathogen interactions, plant hormone signal transduction, MAPK signaling pathways, phenylpropanoid biosynthesis, and phenylalanine metabolism. RT–qPCR was used to verify the expression changes of 12 DEGs, the results of which were consistent with the RNA-seq analysis results. The expression of DEGs related to the MAPK pathway were significantly different between the MB group and the HB group, suggesting that, via the MAPK signaling cascade, melatonin may play a role in the disease resistance of Lilium to B. elliptica. This study provides a new perspective and information for molecular-based breeding of Lilium disease resistance.
Keywords
