Extracellular-Added ADP-Ribose Increases Intracellular Free Ca2+ Concentration Through Ca2+ Release From Stores, but Not Through TRPM2-Mediated Ca2+ Entry, in Rat β-Cell Line RIN-5F

Ishii Masakazu; Shimizu Shunichi; Hagiwara Tamio; Wajima Teruaki; Miyazaki Akira; Mori Yasuo; Kiuchi Yuji

Journal of Pharmacological Sciences (Jan 2006)

Extracellular-Added ADP-Ribose Increases Intracellular Free Ca2+ Concentration Through Ca2+ Release From Stores, but Not Through TRPM2-Mediated Ca2+ Entry, in Rat β-Cell Line RIN-5F

Ishii Masakazu,
Shimizu Shunichi,
Hagiwara Tamio,
Wajima Teruaki,
Miyazaki Akira,
Mori Yasuo,
Kiuchi Yuji

Affiliations

Ishii Masakazu: Department of Pathophysiology, School of Pharmaceutical Sciences, Showa University, Shinagawa-ku, Tokyo 142-8555, Japan; Corresponding author. [email protected]
Shimizu Shunichi: Department of Pathophysiology, School of Pharmaceutical Sciences, Showa University, Shinagawa-ku, Tokyo 142-8555, Japan
Hagiwara Tamio: Department of Biochemistry, Showa University School of Medicine, Shinagawa-ku, Tokyo 142-8555, Japan
Wajima Teruaki: Department of Pathophysiology, School of Pharmaceutical Sciences, Showa University, Shinagawa-ku, Tokyo 142-8555, Japan; Laboratory of Molecular Biology, Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan
Miyazaki Akira: Department of Biochemistry, Showa University School of Medicine, Shinagawa-ku, Tokyo 142-8555, Japan
Mori Yasuo: Laboratory of Molecular Biology, Department of Synthetic and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan
Kiuchi Yuji: Department of Pathophysiology, School of Pharmaceutical Sciences, Showa University, Shinagawa-ku, Tokyo 142-8555, Japan

Journal volume & issue: Vol. 101, no. 2
pp. 174 – 178

Abstract

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Abstract.: Intracellular ADP-ribose is an activator of TRPM2, which is a Ca2+-permeable channel and mediates H2O2-induced cell death, in the TRPM2-expressing rat β-cell line RIN-5F. We examined the effect of extracellular-added ADP-ribose on intracellular Ca2+ concentration in RIN-5F cells. ADP-ribose induced Ca2+ release from the thapsigargin-sensitive Ca2+ store, but not Ca2+ entry across the plasma membrane. A phospholipase C (PLC) inhibitor and a non-specific IP3 receptor inhibitor blocked its Ca2+ release. H2O2-induced Ca2+ entry through TRPM2 was not affected by extracellular ADP-ribose. These findings suggest that extracellular-added ADP-ribose induces Ca2+ release through the PLC-IP3 pathway and does not act as a TRPM2 activator. Keywords:: ADP-ribose, hydrogen peroxide, TRPM2

Published in Journal of Pharmacological Sciences

ISSN: 1347-8613 (Print)
Publisher: Elsevier
Country of publisher: Japan
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.journals.elsevier.com/journal-of-pharmacological-sciences

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