Screening and nitrogen removal characteristics of a denitrifying bacteria suitable for partial denitrification

Abstract

A strain D5 with excellent partial denitrification performance was screened from paddy soil. The bacteria were identified as Klebsiella pneumoniae by morphological observation and 16S r DNA sequence analysis. The denitrification ability of strain D5 under different environmental factors （carbon source，C/N ratio and temperature） were investigated using single-factor experiments. The results showed that various environmental factors had obvious influence on partial denitrification of strain D5. The optimal conditions for partial denitrification activity of strain D5 were carbon source of sodium citrate， C/N ratio of 3，temperatures of 25 ℃ and 35 ℃. The results showed that strain D5 had excellent partial denitrification performance，and the optimal partial denitrification effect could be achieved by regulating environmental conditions.

Keywords

• denitrifying bacteria
• partial denitrification
• klebsiella pneumoniae
• carbon source