Nature Communications (Mar 2022)
Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure
- Xiaosa Li,
- Lina Zhou,
- Bao-Qing Gao,
- Guangye Li,
- Xiao Wang,
- Ying Wang,
- Jia Wei,
- Wenyan Han,
- Zixian Wang,
- Jifang Li,
- Runze Gao,
- Junjie Zhu,
- Wenchao Xu,
- Jing Wu,
- Bei Yang,
- Xiaodong Sun,
- Li Yang,
- Jia Chen
Affiliations
- Xiaosa Li
- Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine
- Lina Zhou
- Gene Editing Center, School of Life Science and Technology, ShanghaiTech University
- Bao-Qing Gao
- CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences
- Guangye Li
- Gene Editing Center, School of Life Science and Technology, ShanghaiTech University
- Xiao Wang
- Gene Editing Center, School of Life Science and Technology, ShanghaiTech University
- Ying Wang
- CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences
- Jia Wei
- CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences
- Wenyan Han
- Gene Editing Center, School of Life Science and Technology, ShanghaiTech University
- Zixian Wang
- Gene Editing Center, School of Life Science and Technology, ShanghaiTech University
- Jifang Li
- Gene Editing Center, School of Life Science and Technology, ShanghaiTech University
- Runze Gao
- Gene Editing Center, School of Life Science and Technology, ShanghaiTech University
- Junjie Zhu
- Gene Editing Center, School of Life Science and Technology, ShanghaiTech University
- Wenchao Xu
- Gene Editing Center, School of Life Science and Technology, ShanghaiTech University
- Jing Wu
- Gene Editing Center, School of Life Science and Technology, ShanghaiTech University
- Bei Yang
- Shanghai Clinical Research and Trial Center
- Xiaodong Sun
- Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine
- Li Yang
- Center for Molecular Medicine, Children’s Hospital, Fudan University and Shanghai Key Laboratory of Medical Epigenetics, International Laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University
- Jia Chen
- Gene Editing Center, School of Life Science and Technology, ShanghaiTech University
- DOI
- https://doi.org/10.1038/s41467-022-29339-9
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 9
Abstract
Prime editors can mediate all twelve types of base substitutions and small insertions or deletions in living cells but its efficiency remains low. Here the authors introduce same-sense mutations into pegRNAs to increase base-editing efficiency and the pegRNA secondary structure was altered to increase indel-editing efficiency.
