CRL4Wdr70 regulates H2B monoubiquitination and facilitates Exo1-dependent resection

Ming Zeng; Laifeng Ren; Ken'Ichi Mizuno; Konstantinos Nestoras; Haibin Wang; Zizhi Tang; Liandi Guo; Daochun Kong; Qiwen Hu; Qun He; Lilin Du; Antony M. Carr; Cong Liu

doi:10.1038/ncomms11364

Nature Communications (Apr 2016)

CRL4Wdr70 regulates H2B monoubiquitination and facilitates Exo1-dependent resection

Ming Zeng,
Laifeng Ren,
Ken'Ichi Mizuno,
Konstantinos Nestoras,
Haibin Wang,
Zizhi Tang,
Liandi Guo,
Daochun Kong,
Qiwen Hu,
Qun He,
Lilin Du,
Antony M. Carr,
Cong Liu

Affiliations

Ming Zeng: Department of Paediatrics, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), West China Second University Hospital, Sichuan University
Laifeng Ren: Department of Medical Microbiology and Immunology, Fenyang College of Shanxi Medical University
Ken'Ichi Mizuno: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Konstantinos Nestoras: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Haibin Wang: Department of Paediatrics, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), West China Second University Hospital, Sichuan University
Zizhi Tang: Department of Paediatrics, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), West China Second University Hospital, Sichuan University
Liandi Guo: Department of Paediatrics, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), West China Second University Hospital, Sichuan University
Daochun Kong: National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University
Qiwen Hu: Department of Microbiology, College of Basic Medical Sciences, Third Military Medical University
Qun He: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University
Lilin Du: National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park
Antony M. Carr: Genome Damage and Stability Centre, School of Life Sciences, University of Sussex
Cong Liu: Department of Paediatrics, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), West China Second University Hospital, Sichuan University

DOI: https://doi.org/10.1038/ncomms11364
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 11

Abstract

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The repair of double-strand DNA breaks by homologous recombination requires resection of the DNA ends. Here the authors show that in Schizosaccharomyces pombe and human cells, Wdr70 is recruited as part of the CRL4 complex to promote ubiquitination of H2B and allow Exo1-mediated resection.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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