Genetic polymorphism of glutathione s-transferase M1 (GSTM1) in patients with susceptible and resistant tuberculosis

Abstract

The objective of the study. To identify glutathione S-transferase M1 (GSTМ1) gene polymorphism in patients with tuberculosis depending on Mycobacterium tuberculosis (MBT) resistance. Material and methods. The study involved 100 patients with newly diagnosed pulmonary tuberculosis (TB), who had been hospitalized in Chernivtsi Regional TB Dispensary. The control group consisted of 50 healthy individuals. Genomic DNA was isolated from the whole venous blood. GSTM1 polymorphic areas were isolated by means of multicomplex polymerase chain reaction, according to the protocol for instantaneous analysis of polymorphism by Arana et al (1996). Deletion of gene corresponds to the lack of appropriate strips in the electropherogram. Results. An analysis of the occurrence of alleles and genotypes of GSTM1 gene in patients with pulmonary tuberculosis regarding the MBT resistance version allowed to establish that, under the conditions of pulmonary tuberculosis infection, GSTM1 gene deletion mutation can be found in one out of five (21.87% of cases), and the occurrence due to the MBT resistance version is: with newly diagnosed tuberculosis (NDTB) – 17.39%, with multidrug-resistant tuberculosis (MDR-TB) 35.0% and polyresistant pulmonary tuberculosis (PRTB) 20.0%, respectively. According to the nature of the distribution of allelic gene GSTM1, a favorable functional 1 allele prevails (73.29%) in the normal inbreeding among patients and deficiency of heterozygosity among healthy people, which generally forms a normal population distribution for the European race. Conclusions. According to the nature of allele distribution of GSTM1 gene, the favorable functional 1 allele prevails (73.29%) in case of normal inbreeding in patients (F=0,20, р>0,05) and lack of heterozygote in healthy individuals (F=0.28 р=0.033), which in general forms a normal population distribution [OR=14.06, р=0.005].

Keywords

• tuberculosis
• deletion polymorphism
• glutathione s-transferase M1
• resistance
• Mycobacterium tuberculosis