ROS-responsive capsules engineered from EGCG-Zinc networks improve therapeutic angiogenesis in mouse limb ischemia

Zuoguan Chen; Jianwei Duan; Yongpeng Diao; Youlu Chen; Xiaoyu Liang; Huiyang Li; Yuqing Miao; Qing Gao; Liang Gui; Xiaoli Wang; Jing Yang; Yongjun Li

Bioactive Materials (Jan 2021)

ROS-responsive capsules engineered from EGCG-Zinc networks improve therapeutic angiogenesis in mouse limb ischemia

Zuoguan Chen,
Jianwei Duan,
Yongpeng Diao,
Youlu Chen,
Xiaoyu Liang,
Huiyang Li,
Yuqing Miao,
Qing Gao,
Liang Gui,
Xiaoli Wang,
Jing Yang,
Yongjun Li

Affiliations

Zuoguan Chen: Department of Vascular Surgery, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine. Chinese Academy of Medical Sciences and Peking Union Medical College. Graduate School of Peking Union Medical College, Beijing, 100730, PR China
Jianwei Duan: Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300192, PR China
Yongpeng Diao: Department of Vascular Surgery, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine. Chinese Academy of Medical Sciences and Peking Union Medical College. Graduate School of Peking Union Medical College, Beijing, 100730, PR China
Youlu Chen: Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300192, PR China
Xiaoyu Liang: Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300192, PR China
Huiyang Li: Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300192, PR China
Yuqing Miao: Department of Vascular Surgery, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine. Chinese Academy of Medical Sciences and Peking Union Medical College. Graduate School of Peking Union Medical College, Beijing, 100730, PR China
Qing Gao: Department of Vascular Surgery, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine. Chinese Academy of Medical Sciences and Peking Union Medical College. Graduate School of Peking Union Medical College, Beijing, 100730, PR China
Liang Gui: Department of Vascular Surgery, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine. Chinese Academy of Medical Sciences and Peking Union Medical College. Graduate School of Peking Union Medical College, Beijing, 100730, PR China
Xiaoli Wang: Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300192, PR China
Jing Yang: Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300192, PR China; Corresponding author. No. 1 Dahua Road, Dong Dan, Beijing, 100730, PR China.
Yongjun Li: Department of Vascular Surgery, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine. Chinese Academy of Medical Sciences and Peking Union Medical College. Graduate School of Peking Union Medical College, Beijing, 100730, PR China; Corresponding author.

Journal volume & issue: Vol. 6, no. 1
pp. 1 – 11

Abstract

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The successful treatment of limb ischemia requires that promote angiogenesis along with microenvironment improvement. Zinc ions have been reported to stimulate angiogenesis, but application was limited to the toxicity concerns. We hypothesized that zinc based metal-EGCG capsule (EGCG/Zn Ps) can achieve sustained release Zn2+ resulting in reduced toxicity and improve angiogenesis as well as the improvement of microenvironment by ROS scavenging of EGCG. The surface morphology, zeta potential, infrared absorbance peaks and zinc ion release profile of the EGCG/Zn Ps were measured. In vitro, EGCG/Zn showed significantly antioxidant, anti-inflammatory and induced cell migration effect. In addition, EGCG/Zn Ps enabled the sustained release of zinc ions, which reduced cytotoxicity and enhanced the secretion of vascular endothelial growth factor (VEGF) in vitro and in vivo. In mouse models of limb ischemia, EGCG/Zn Ps promoted angiogenesis and cell proliferation in ischemic tissues. Moreover, EGCG/Zn Ps group exhibited the most significant recovery of limb ischemic score, limb temperature and blood flow than other groups. In conclusion, EGCG/Zn Ps is a safe and promising approach to combine the merit of Zn2+ and EGCG, thus enabling the direct application to limb ischemia.

Published in Bioactive Materials

ISSN: 2452-199X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials; Science: Biology (General)
Website: https://www.keaipublishing.com/en/journals/bioactive-materials/

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