پزشکی بالینی ابن سینا (Mar 2001)
The Evaluation of Evolutional Estradiol-Indiuced Disorders on Motor Limbs of Rat’s Fetuses
Abstract
Estrogen and progesteron are the most important female sexual hormons which is administrated for the treatment of some women diseases and also are used in contraceptive pills. Administration of female sexual hormons during critical period of embryonic development may produce malformations. In this study which was undertaken during 1998-99 in biology department, school of sciences, Ferdowsi university of Mashhad. The incidence of motor limbs abnormality due to estradiol administration during the rat embryonic development was investigated. Three groups of pregnant wistar rats were exposed to 0.15mg/kg estradiol valerate via a single intramuscular injection on one of the following gestational days;12,14 or 16 of experimental groups 1, 2 and 3 respectively. The control pregnant rats(group 4), were recieved physiological serum in the same manner. All of pregnant rats were sacrificed on the last day of pregnancy and their fetuses were collected, counted and examind for limbs abnormalities. Our results indicate that in comparison with control group, in experimental groups the size of litters significantly reduce and the incidence of malformations, especially in experimental group 1 are significantly high. The markedly limbs malformations are syndactyly , tetraductyly and phocomellya. Since the critical period of rat motor limbs embryonic development is between 11to 13th post-mating day, the estradiol administration in group 1 probably by interrupting the programed cell death phenomena or blocking the chemical receptors in limb bods produce limb abnormalities.
