PrimeDesign software for rapid and simplified design of prime editing guide RNAs

Jonathan Y. Hsu; Julian Grünewald; Regan Szalay; Justine Shih; Andrew V. Anzalone; Kin Chung Lam; Max W. Shen; Karl Petri; David R. Liu; J. Keith Joung; Luca Pinello

doi:10.1038/s41467-021-21337-7

Nature Communications (Feb 2021)

PrimeDesign software for rapid and simplified design of prime editing guide RNAs

Jonathan Y. Hsu,
Julian Grünewald,
Regan Szalay,
Justine Shih,
Andrew V. Anzalone,
Kin Chung Lam,
Max W. Shen,
Karl Petri,
David R. Liu,
J. Keith Joung,
Luca Pinello

Affiliations

Jonathan Y. Hsu: Department of Biological Engineering, Massachusetts Institute of Technology
Julian Grünewald: Molecular Pathology Unit, Massachusetts General Hospital
Regan Szalay: Molecular Pathology Unit, Massachusetts General Hospital
Justine Shih: Molecular Pathology Unit, Massachusetts General Hospital
Andrew V. Anzalone: Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT
Kin Chung Lam: Molecular Pathology Unit, Massachusetts General Hospital
Max W. Shen: Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT
Karl Petri: Molecular Pathology Unit, Massachusetts General Hospital
David R. Liu: Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT
J. Keith Joung: Molecular Pathology Unit, Massachusetts General Hospital
Luca Pinello: Molecular Pathology Unit, Massachusetts General Hospital

DOI: https://doi.org/10.1038/s41467-021-21337-7
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 6

Abstract

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Prime editing guide RNA design is more complex than for standard CRISPR-based nucleases or base editors. Here the authors present PrimeDesign and PrimeVar for the rapid and simplified design of pegRNA and ngRNA combinations.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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