Effects of in ovo feeding of t10,c12-conjugated linoleic acid on hepatic lipid metabolism and subcutaneous adipose tissue deposition in newly hatched broiler chicks

Wentao Xu; Zhigang Song; Wenbin Wang; Xia Li; Peipei Yan; Tianhong Shi; Chunyan Fu; Xuelan Liu

Poultry Science (May 2022)

Effects of in ovo feeding of t10,c12-conjugated linoleic acid on hepatic lipid metabolism and subcutaneous adipose tissue deposition in newly hatched broiler chicks

Wentao Xu,
Zhigang Song,
Wenbin Wang,
Xia Li,
Peipei Yan,
Tianhong Shi,
Chunyan Fu,
Xuelan Liu

Affiliations

Wentao Xu: Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan 250023, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271018, China
Zhigang Song: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271018, China
Wenbin Wang: Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan 250023, China; Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Jinan 250023, China
Xia Li: Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan 250023, China; Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Jinan 250023, China; Poultry Breeding Engineering Technology Center of Shandong Province, Jinan 250023, China
Peipei Yan: Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan 250023, China; Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Jinan 250023, China; Poultry Breeding Engineering Technology Center of Shandong Province, Jinan 250023, China
Tianhong Shi: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271018, China; Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Jinan 250023, China; Poultry Breeding Engineering Technology Center of Shandong Province, Jinan 250023, China
Chunyan Fu: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271018, China; Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Jinan 250023, China; Poultry Breeding Engineering Technology Center of Shandong Province, Jinan 250023, China; Corresponding author:
Xuelan Liu: Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271018, China; Shandong Provincial Key Laboratory of Poultry Diseases Diagnosis and Immunology, Jinan 250023, China; Poultry Breeding Engineering Technology Center of Shandong Province, Jinan 250023, China

Journal volume & issue: Vol. 101, no. 5
p. 101797

Abstract

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ABSTRACT: The purpose of this study was to investigate whether in ovo feeding of t10,c12-conjugated linoleic acid (CLA) could regulate hepatic lipid metabolism and decrease lipid accumulation in newly hatched chicks. Three hundred and sixty fertilely specific pathogen-free hatching eggs were selected and randomly divided into 6 groups. On embryonic day 11 of incubation (E11), 0, 1.5, 3.0, 4.5, 6.0, or 7.5 mg t10,c12-CLA were injected into the eggs. The results indicated that in ovo feeding of t10,c12-CLA significantly decreased the subcutaneous adipose tissue (SAT) mass and the relative SAT weight of newly hatched chicks in linear and quadratic manners (P < 0.05). In liver, the levels of triglycerides were reduced linearly and quadratically and total cholesterol were reduced quadratically as the dose of t10,c12-CLA increased (P < 0.05). Meanwhile, the hepatic carnitine palmitoyltransferase-1a (CPT1a) content and polyunsaturated fatty acid proportion were increased quadratically in t10,c12-CLA groups (P < 0.05), accompanied by the decrease of malondialdehyde level and the increase of glutathione peroxidase and total antioxidant capacity activities (P < 0.05). In addition, in ovo feeding of t10,c12-CLA decreased the mRNA expression levels of fatty acid synthase, acetyl-CoA carboxylase 1 in linear and quadratic manners (P < 0.05), and decreased the mRNA expression of adipose triacylglyceride lipase and stearoyl-CoA desaturase significantly in liver (P < 0.05), accompanied by upregulating the mRNA expression of CPT1a quadratically and AMP-activated protein kinase α linearly and quadratically (P < 0.05). In SAT, the mRNA expression of peroxisome proliferator-activated receptor γ (PPARγ) and sterol regulatory element-binding protein-1c were decreased linearly and quadratically (P < 0.05), and the expression of PPARα and CPT1a genes were increased linearly and quadratically as the dose of t10,c12-CLA increased (P < 0.05). In conclusion, our findings demonstrate that in ovo feeding of t10,c12-CLA alleviates lipid accumulation in newly hatched chicks by suppressing fatty acid synthesis and stimulating lipolysis in the liver and inhibiting adipocyte differentiation in subcutaneous adipose tissue.

Published in Poultry Science

ISSN: 0032-5791 (Print); 1525-3171 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Agriculture: Animal culture
Website: https://www.journals.elsevier.com/poultry-science

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