EVALUATION OF CYTOKINES AND HEAT SHOCK PROTEIN IN VIBRATION DISEASE

Abstract

The prolonged impact of industrial vibration on workers leads to the development of a vibrational disease (VB), which occupies a leading position in the structure of occupational pathology. VB from the impact of local vibration is a chronic occupational disease characterized by a predominant lesion of the nervous, vascular system and musculoskeletal system of the upper and lower extremities. One of the real ways to reduce the incidence is the early detection of the negative impact of vibration on the body of workers. In this regard, cytokines and heat shock proteins (HSP70) can be early and sensitive indicators that reflect the severity of health disorders from exposure to vibration. The aim of the study was to study changes in the content of pro- and antiinflammatory cytokines, extracellular HSP70 and their relationship in patients with VB. In the immunological study included 43 male patients with a diagnosis of VB. The criteria for inclusion in this group were: a verified diagnosis, written informed consent to participate in the study, the harmful effects of local vibration in the workplace. According to the data of hygienic control, the working conditions of workers in dangerous occupations by vibration belong to the 4 (dangerous) class due to intensive local vibration. The content of cytokines: IL-1β, IL-8, IL-10, TNFα and HSP70 in the serum of patients was determined by enzyme immunoassay. The blood sampling for the study was taken from patients only once on admission to the hospital before the treatment. Statistical processing of data was carried out with the help of packages of application programs Statistica for Windows 6.0 and Microsoft Excel. It was established that the development of VB disease is accompanied by imbalance of the cytokine profile, characterized by a decrease in the levels of IL-1β, IL-10 and an increase in IL-8. The inhibition of production of IL-1β and IL-10 is a consequence of the chronic process that has developed in the body of the patients examined. And the revealed decrease in extracellular concentration of HSP70 in comparison with practically healthy people can be caused by the accumulation of it inside the cell or on its surface. Correlation analysis revealed the relationship between a decrease in HSP70 and an increase in IL-1β, and a decrease in IL-10 levels. Synthesis of HSP is an intracellular defense mechanism that prevents cell damage by activating the synthesis and secretion of pro-inflammatory cytokines. The obtained relationships between cytokines and HSP70 testify to the involvement of HSP70 in immunoinflammatory processes in VB. The revealed changes contribute to the chronic inflammatory process and justify the progressive course of the VB.

Keywords

• cytokines
• heat shock protein 70
• vibration disease
• immune response