Biological control of Root-knot nematode (Meloidogyne javanica) by potential antagonism of endophytic fungi isolated from Taify roses

Abstract

Objectives: Plant-parasitic nematodes are a serious threat to global agricultural production worldwide. To avoid synthetic chemistries toxicity there is a dire need of the hour to develop innovative nematode control strategies. Biological control using the antagonistic fungi of the plants is considered very economical and environment friendly. Methods: Efficacy of different fungal filtrate concentrations was estimated towards the mortality and clutching of Meloidogyne javanica. To determine the antagonistic potential of Penicillium citrinum (MN518391), time of disclosure was assessed in the laboratory with the result “exposure to 8% filtrate for a clock (24 hrs.), expressively reduced the viable juveniles and hatched eggs. Results: Exposition to aforementioned filtrate of P. citrinum for a clock (24 hrs.) revealed that juvenile body turned into straight stature leading to reduction in viability of nematodes. Contrary to it most of nematodes exhibited bent body when exposed to Aspergillusniger (MK713445), or (MN513383) its filtrates. Among the various isolated antagonistic fungi, GC–MS analysis revealed 22 unique compounds e.g., quinazoline and its derivatives as the most prevalent and squalene as least occurring possess significant nematicidal properties against the rootknot nematodes. Conclusion: Hence, using antagonistic fungus from the described plans as BCAs in agriculture against plant-parasitic nematodes is a viable long-term biocontrol technique and provide future avenues in this area of research.

Keywords

• Obligate parasites
• Small animals
• Bio-control
• Meloidogyne javanica
• Bioactive compounds