Identification of transition factors in myotube formation from proteome and transcriptome analyses

Qi ZHENG; Rong-cui HU; Cui-yun ZHU; Jing JING; Meng-yu LOU; Si-huan ZHANG; Shuang LI; Hong-guo CAO; Xiao-rong ZHANG; Ying-hui LING

Journal of Integrative Agriculture (Oct 2023)

Identification of transition factors in myotube formation from proteome and transcriptome analyses

Qi ZHENG,
Rong-cui HU,
Cui-yun ZHU,
Jing JING,
Meng-yu LOU,
Si-huan ZHANG,
Shuang LI,
Hong-guo CAO,
Xiao-rong ZHANG,
Ying-hui LING

Affiliations

Qi ZHENG: College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R.China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, P.R.China
Rong-cui HU: College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R.China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, P.R.China
Cui-yun ZHU: College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R.China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, P.R.China
Jing JING: College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R.China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, P.R.China
Meng-yu LOU: College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R.China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, P.R.China
Si-huan ZHANG: College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R.China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, P.R.China
Shuang LI: College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R.China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, P.R.China
Hong-guo CAO: College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R.China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, P.R.China
Xiao-rong ZHANG: College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R.China; Key Laboratory of Embryo Development and Reproductive Regulation of Anhui Province, Fuyang Normal University, Fuyang 236041, P.R.China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, P.R.China
Ying-hui LING: College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, P.R.China; Key Laboratory of Embryo Development and Reproductive Regulation of Anhui Province, Fuyang Normal University, Fuyang 236041, P.R.China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, P.R.China; Correspondence LING Ying-hui

Journal volume & issue: Vol. 22, no. 10
pp. 3135 – 3147

Abstract

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Muscle fibers are the main component of skeletal muscle and undergo maturation through the formation of myotubes. During early development, a population of skeletal muscle satellite cells (SSCs) proliferate into myoblasts. The myoblasts then undergo further differentiation and fusion events, leading to the development of myotubes. However, the mechanisms involved in the transition from SSCs to myotube formation remain unclear. In this study, we characterized changes in the proteomic and transcriptomic expression profiles of SSCs, myoblasts (differentiation for 2 d) and myotubes (differentiation for 10 d). Proteomic analysis identified SLMAP and STOM as potentially associated with myotube formation. In addition, some different changes in MyoD, MyoG, Myosin7 and Desmin occurred after silencing SLMAP and STOM, suggesting that they may affect changes in the myogenic marker. GO analysis indicated that the differentiation and migration factors SVIL, ENSCHIG00000026624 (AQP1) and SERPINE1 enhanced the transition from SSCs to myoblasts, accompanied by changes in the apoptotic balance. In the myoblast vs. myotube group, candidates related to cell adhesion and signal transduction were highly expressed in the myotubes. Additionally, CCN2, TGFB1, MYL2 and MYL4 were identified as hub-candidates in this group. These data enhance our existing understanding of myotube formation during early development and repair.

Published in Journal of Integrative Agriculture

ISSN: 2095-3119 (Print); 2352-3425 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Agriculture (General)
Website: https://www.journals.elsevier.com/journal-of-integrative-agriculture

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