Effects of high choline diet on serum amino acids profile in mice

Abstract

ObjectiveTo establish a high choline diet mouse model and investigate the effect of high choline diet on serum amino acid metabolism in mice.MethodsTwenty SPF-grade 4-week-old mice were randomly divided into two groups， the control group was given ordinary mouse food and laboratory drinking water， and the high-choline diet group were given 3% choline aqueous solution instead of daily drinking water. Serum was collected after 8 weeks of feeding， and serum amino acids levels were quantified by ultra-performance liquid chromatography tandem triple quadrupole mass spectrometry （UPLC-MS/MS）. Principal component analysis and orthogonal partial least squares-discriminant analysis were used to determine the similarity of amino acid composition between the high choline diet group and the control group.ResultsCompared with the control group， the levels of cystine， serine， alanine， glutamic acid， phenylalanine， asparagine， threonine， proline， trans-4-hydroxyproline and branched-chain amino acids were significantly higher and the levels of lysine were significantly lower in the high-choline diet group， with isoleucine levels of 34.20±2.71 and 72.59±10.42 µmol/L； the levels of leucine were 68.59±4.06 and 113.04±14.64 µmol/L； the levels of valine were 129.86±10.54 and 161.95±16.54 µmol/L， respectively， and the differences between groups were statistically significant （P<0.05）.ConclusionThe high choline diet cause significant changes in serum amino acid metabolism in mice.

Keywords

• high choline diet
• amino acids
• metabolism of amino acids
• ultra-high liquid chromatography tandem mass spectrometry