Systemic nanoparticle delivery of CRISPR-Cas9 ribonucleoproteins for effective tissue specific genome editing

Tuo Wei; Qiang Cheng; Yi-Li Min; Eric N. Olson; Daniel J. Siegwart

doi:10.1038/s41467-020-17029-3

Nature Communications (Jun 2020)

Systemic nanoparticle delivery of CRISPR-Cas9 ribonucleoproteins for effective tissue specific genome editing

Tuo Wei,
Qiang Cheng,
Yi-Li Min,
Eric N. Olson,
Daniel J. Siegwart

Affiliations

Tuo Wei: Department of Biochemistry, Simmons Comprehensive Cancer Center, The University of Texas Southwestern Medical Center
Qiang Cheng: Department of Biochemistry, Simmons Comprehensive Cancer Center, The University of Texas Southwestern Medical Center
Yi-Li Min: Department of Molecular Biology, Hamon Center for Regenerative Science and Medicine, The University of Texas Southwestern Medical Center
Eric N. Olson: Department of Molecular Biology, Hamon Center for Regenerative Science and Medicine, The University of Texas Southwestern Medical Center
Daniel J. Siegwart: Department of Biochemistry, Simmons Comprehensive Cancer Center, The University of Texas Southwestern Medical Center

DOI: https://doi.org/10.1038/s41467-020-17029-3
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 12

Abstract

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Therapeutic targets of CRISPR-Cas can often not be accessed due to lack of carriers to deliver RNPs systematically. Here, the authors engineer modified lipid nanoparticles for delivery of gene editing proteins to specific tissues.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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