Serological and Molecular identification of Zucchini yellow mosaic virus in Khorasan Razavi province

Abstract

Zucchini yellow mosaic virus (ZYMV) causes high yield losses to cucurbits in many parts of the world. During a survey in summer and fall of 2008 and 2009, 392 collected from different fields in Khorasan Razavi, Northern Khorasan and Southern Khorasan provinces were tested based on DAS-ELISA tests using ZYMV specific polyclonal antibody. The result showed that 72 out of 392 samples were infected by ZYMV. Subsequently two ELISA positive samples were selected based on host range and sampling location for further investigation using specific primers for ZYMV coat protein gene. A fragment (970bp) was amplified in reverse transcription polymerase chain reaction (RT-PCR) using total RNA of plants infected with isolates of ZYMV(P03,Da). Phylogenetic analysis showed that Neyshabour(Po3) and Dargaz (Da) isolate were very close up to 94% identity in cp gene to SYZY-30 isolate from Syria.

Keywords

• ZYMV
• DAS-ELISA
• Polymerase Chain Reaction
• Phylogenetic analysis