Structural heterogeneity of α-synuclein fibrils amplified from patient brain extracts

Timo Strohäker; Byung Chul Jung; Shu-Hao Liou; Claudio O. Fernandez; Dietmar Riedel; Stefan Becker; Glenda M. Halliday; Marina Bennati; Woojin S. Kim; Seung-Jae Lee; Markus Zweckstetter

doi:10.1038/s41467-019-13564-w

Nature Communications (Dec 2019)

Structural heterogeneity of α-synuclein fibrils amplified from patient brain extracts

Timo Strohäker,
Byung Chul Jung,
Shu-Hao Liou,
Claudio O. Fernandez,
Dietmar Riedel,
Stefan Becker,
Glenda M. Halliday,
Marina Bennati,
Woojin S. Kim,
Seung-Jae Lee,
Markus Zweckstetter

Affiliations

Timo Strohäker: German Center for Neurodegenerative Diseases (DZNE)
Byung Chul Jung: Department of Biomedical Sciences, Neuroscience Research Institute, College of Medicine, Seoul National University
Shu-Hao Liou: RG EPR Spectroscopy, Max Planck Institute for Biophysical Chemistry
Claudio O. Fernandez: Max Planck Laboratory for Structural Biology, Chemistry and Molecular Biophysics of Rosario (MPLbioR, UNR-MPIbpC)
Dietmar Riedel: Department for Electron Microscopy, Max Planck Institute for Biophysical Chemistry
Stefan Becker: Department for NMR-based Structural Biology, Max Planck Institute for Biophysical Chemistry
Glenda M. Halliday: Brain and Mind Centre and Central Clinical School, Faculty of Medicine and Health, The University of Sydney
Marina Bennati: RG EPR Spectroscopy, Max Planck Institute for Biophysical Chemistry
Woojin S. Kim: Brain and Mind Centre and Central Clinical School, Faculty of Medicine and Health, The University of Sydney
Seung-Jae Lee: Department of Biomedical Sciences, Neuroscience Research Institute, College of Medicine, Seoul National University
Markus Zweckstetter: German Center for Neurodegenerative Diseases (DZNE)

DOI: https://doi.org/10.1038/s41467-019-13564-w
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 12

Abstract

Read online

Parkinson’s disease (PD) and Multiple System Atrophy (MSA) are characterized by the pathological accumulation of α-synuclein. Here the authors employ fluorescent probes, electron microscopy and NMR spectroscopy to study the properties of α-synuclein aggregates that were amplified from patient brain extracts and observe a greater structural diversity among PD patients compared to MSA patients.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal