Use of constant composition polyvinylpyrrolidone columns to study the interaction of fat particles with plasma

Abstract

Fat particles (lipoproteins of Sf > 400) have been obtained from dog and human lymph and from human post-alimentary plasma. They were flocculated by a solution of 5% polyvinylpyrrolidone (PVP) in 10% NaCl and layered at the bottom of tubes of PVP of various concentrations between 2% and 5% (w/v). As the concentration of PVP increased, a greater proportion of the particles accumulated at the top of the tube (``primary'' particles). When the concentration of PVP in the tube was held constant at 3% PVP, dilution of the sample with plasma was found to produce an increased proportion of bottom particles (``secondary'' particles). This observation suggests that bottom particles result from an interaction of top particles with plasma.

Keywords

• dog
• man
• lymph
• plasma
• primary
• secondary