Generation of an induced pluripotent stem cell line from a Chinese Han child with arrhythmia

Cuilan Hou; Wei Liu; Lijian Xie; Junmin Zheng; Xiaonan Chen; Xiaomin Sun; Yongwei Zhang; Meng Xu; Yun Li; Tingting Xiao

Stem Cell Research (Mar 2021)

Generation of an induced pluripotent stem cell line from a Chinese Han child with arrhythmia

Cuilan Hou,
Wei Liu,
Lijian Xie,
Junmin Zheng,
Xiaonan Chen,
Xiaomin Sun,
Yongwei Zhang,
Meng Xu,
Yun Li,
Tingting Xiao

Affiliations

Cuilan Hou: Department of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, 200062, China; NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology, Shanghai Key Laboratory of Embryo and Reproduction Engineering, Shanghai 200062, China
Wei Liu: Department of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, 200062, China
Lijian Xie: Department of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, 200062, China
Junmin Zheng: Department of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, 200062, China
Xiaonan Chen: Department of Cardiology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200062, China
Xiaomin Sun: Department of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, 200062, China
Yongwei Zhang: Department of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, 200062, China
Meng Xu: Department of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, 200062, China
Yun Li: Department of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, 200062, China; NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology, Shanghai Key Laboratory of Embryo and Reproduction Engineering, Shanghai 200062, China; Corresponding authors at: Department of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, No. 355 Luding Road, Shanghai 200062, China.
Tingting Xiao: Department of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, 200062, China; NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology, Shanghai Key Laboratory of Embryo and Reproduction Engineering, Shanghai 200062, China; Corresponding authors at: Department of Cardiology, Shanghai Children’s Hospital, Shanghai Jiaotong University, No. 355 Luding Road, Shanghai 200062, China.

Journal volume & issue: Vol. 51
p. 102183

Abstract

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CTNNA3, first reported in association with arrhythmogenic right ventricular cardiomyopathy in 2003, is an unique component of both desmosomes and adherens junctions. Using Sendaivirus-mediated reprogramming, we generated an induced pluripotent stem cell (iPSC) line from the peripheral blood mononuclear cells of a child with arrhythmia. The iPSCs exhibited stable amplification, expressed pluripotent markers, and differentiated spontaneously into three germ layers in vitro. Additionally, this iPSC line was found to maintain a normal karyotype and retain the pathogenic mutation in CTNNA3, facilitating a platform to study the disease mechanisms of arrhythmia and dysfunctions related to CTNNA3 mutations.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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