The effect of heat and disinfectants on the viability of infectious bursal disease virus

Abstract

Infectious Bursal Disease (IBD) has been reported in Indonesia since 1983 and has become an endemic disease. IBD virus is known to be quite resistant to physical and chemical reagents compared to other viruses, causing this disease is hard to be eradicated. This study aims to evaluate the effect of heat and disinfectants on the viability of the IBD virus. This study was conducted using a local isolate of IBDV obtained from IRCVS. The virus was exposed to heat and disinfectant. Heat treatment was conducted by exposing the virus to 560C and 600C for 30, 60, 120, and 300 minutes. Similarly, the virus was also treated with two disinfectants, virkon and sodium hypochlorite (bleach) for 30, 60, 120, and 300 minutes with different concentrations. Results showed that the virus can be inactivated at a temperature of 800C and 560C for 120 and 300 minutes, respectively. Virkon at a concentration of 1:200 and 1:400 was able to inactivate the virus at 30, 60, 120, and 300 minutes, while sodium hypochlorite 0.5% requires at least 60 minutes to inactivate the virus.