Lensed Fiber-Optic Two-Photon Endomicroscopy for Field-of-View Enhancement
Conghao Wang,
Huilan Liu,
Jianrui Ma,
Qiang Fu,
Yijun Li,
Yanchuan Chen,
Yuqian Gao,
Jingquan Tian,
Xinlei Luo,
Fei Yu,
Chunzhu Zhao,
Runlong Wu,
Aimin Wang,
Lishuang Feng
Affiliations
Conghao Wang
School of Instrumentation and Optoelectronic Engineering, Beihang University, Beijing 100191, China
Huilan Liu
School of Instrumentation and Optoelectronic Engineering, Beihang University, Beijing 100191, China
Jianrui Ma
School of Instrumentation and Optoelectronic Engineering, Beihang University, Beijing 100191, China
Qiang Fu
Beijing Transcend Vivoscope Biotech Co., Ltd., Beijing 100049, China
Yijun Li
Beijing Transcend Vivoscope Biotech Co., Ltd., Beijing 100049, China
Yanchuan Chen
Beijing Transcend Vivoscope Biotech Co., Ltd., Beijing 100049, China
Yuqian Gao
Beijing Transcend Vivoscope Biotech Co., Ltd., Beijing 100049, China
Jingquan Tian
Beijing Transcend Vivoscope Biotech Co., Ltd., Beijing 100049, China
Xinlei Luo
Beijing Transcend Vivoscope Biotech Co., Ltd., Beijing 100049, China
Fei Yu
Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China
Chunzhu Zhao
National Biomedical Imaging Center, State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing 100871, China
Runlong Wu
National Biomedical Imaging Center, State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing 100871, China
Aimin Wang
State Key Laboratory of Advanced Optical Communication System and Networks, School of Electronics, Peking University, Beijing 100871, China
Lishuang Feng
School of Instrumentation and Optoelectronic Engineering, Beihang University, Beijing 100191, China
Two-photon endomicroscopy is a promising technique with the ability to achieve in situ imaging and diagnosis at subcellular resolution. The large field-of-view capability is essential and useful to locate and image suspicious areas of biological tissue. In this work, we report objective-lens-free, lensed fiber-optic two-photon endomicroscopy for field-of-view enhancement. The field of view of this two-photon endomicroscopic probe is 750 μm with a resolution of 3.03 μm. This 1.6 g miniature probe has an integrated outer diameter of 5.8 mm and a rigid length of 33.5 mm. The imaging performance of the lensed-fiber-optic two-photon endomicroscopy was validated by examining an ex vivo mouse heart, kidney, brain, stomach wall tissues, and in vivo brain tissue.
