Resveratrol Mitigates Metabolism in Human Microglia Cells
Luise Schlotterose,
Mariya S. Pravdivtseva,
Frowin Ellermann,
Olav Jansen,
Jan-Bernd Hövener,
Frank D. Sönnichsen,
François Cossais,
Ralph Lucius,
Kirsten Hattermann
Affiliations
Luise Schlotterose
Institute of Anatomy, Kiel University, 24118 Kiel, Germany
Mariya S. Pravdivtseva
Section Biomedical Imaging, Molecular Imaging North Competence Center (MOIN CC), Department of Radiology and Neuroradiology, University Medical Center Schleswig-Holstein (UKSH), Kiel University, 24105 Kiel, Germany
Frowin Ellermann
Section Biomedical Imaging, Molecular Imaging North Competence Center (MOIN CC), Department of Radiology and Neuroradiology, University Medical Center Schleswig-Holstein (UKSH), Kiel University, 24105 Kiel, Germany
Olav Jansen
Department of Radiology and Neuroradiology, University Medical Center Schleswig-Holstein, 24105 Kiel, Germany
Jan-Bernd Hövener
Section Biomedical Imaging, Molecular Imaging North Competence Center (MOIN CC), Department of Radiology and Neuroradiology, University Medical Center Schleswig-Holstein (UKSH), Kiel University, 24105 Kiel, Germany
Frank D. Sönnichsen
Otto Diels Institute for Organic Chemistry, Kiel University, 24118 Kiel, Germany
François Cossais
Institute of Anatomy, Kiel University, 24118 Kiel, Germany
Ralph Lucius
Institute of Anatomy, Kiel University, 24118 Kiel, Germany
Kirsten Hattermann
Institute of Anatomy, Kiel University, 24118 Kiel, Germany
The recognition of the role of microglia cells in neurodegenerative diseases has steadily increased over the past few years. There is growing evidence that the uncontrolled and persisting activation of microglial cells is involved in the progression of diseases such as Alzheimer’s or Parkinson’s disease. The inflammatory activation of microglia cells is often accompanied by a switch in metabolism to higher glucose consumption and aerobic glycolysis. In this study, we investigate the changes induced by the natural antioxidant resveratrol in a human microglia cell line. Resveratrol is renowned for its neuroprotective properties, but little is known about its direct effect on human microglia cells. By analyzing a variety of inflammatory, neuroprotective, and metabolic aspects, resveratrol was observed to reduce inflammasome activity, increase the release of insulin-like growth factor 1, decrease glucose uptake, lower mitochondrial activity, and attenuate cellular metabolism in a 1H NMR-based analysis of whole-cell extracts. To this end, studies were mainly performed by analyzing the effect of exogenous stressors such as lipopolysaccharide or interferon gamma on the metabolic profile of microglial cells. Therefore, this study focuses on changes in metabolism without any exogenous stressors, demonstrating how resveratrol might provide protection from persisting neuroinflammation.
