Armaghane Danesh Bimonthly Journal (May 2014)
Evaluation of Protective Effects of Crocin Onembyo Developing Process in in Vitro Fertilization (IVF)in Cyclophosphamide Treated Mice
Abstract
Abstract Background & aim: Cyclophosphamide (CP) along with therapeutic role, exert the effects such as an immunosuppressant agent, induce oxidative stress, impact gonadal cell’s DNA and reducing the fertilizing potential. The present study was aimed to evaluate the protective effects of crocin, on IVF embryos development following chemotherapy with CP. Methods: In this study 36 adult female mice, aged 6-8 weeks, divided into 3 groups and treared for 21 days. The control animals received saline normal (0.1ml/day, IP), and sham control group received CP alone (15 mg/kg/week, IP) and experimental group received crocin (200mg/kg/day, IP) along with CP (15 mg/kg/week, IP). After the end of treatment period PMSG and HCG were administrated for stimulating the ovulation process. The sperms were obtained from 6 adult male mice. Following the euthanizing of animals, and oocyte along with normal sperm collection in-vitro fertilizing was performed by using HTF+4mg/ml BSA medium. The fertilized oocytes were incubated for 120 hours and embryos were studied in various stages. All data were analyzed statistically by Minitab software. Results: In sham control group a significantly reduction in suitable oocytes, percentage of fertilization, two cells zygote, blastocysts and increase the number of arrested developing embryos observed in comparison to control group, and administration of crocin caused significantly increase in suitable oocytes, percentage of fertilization, two cells zygote, blastocysts and significantly reduction of arrested developing embryos in comparison to sham control group (P<0.05). Conclusion: The co-administration of crocin with CP chemotherapy caused a significant improvement in fertilizing potential and promoted the embryo development. Key words: Cyclophosphamide, Crocin, Mice, Oocyte, In vitro fertilizing
