Bihdād (Jun 2019)
Investigation of Serum Level and IL-13 Genetic Variations in Positions +2044 G/A and -1512A/C in Southern Iranian Patients with H. Pylori
Abstract
Background: Helicobacter pylori (H. pylori)is agramnegative bacterium thatcolonizes stomach mucosa andcausesupper gastrointestinal diseases.Interleukin-13 is a preventive immune cytokine produced by many immune cells. IL-13 gene is located on chromosome 5 q 25 bp. It has polymorphisms at positions -1512 A/ C and +2044 G/A, which are associated with the expression rate of this cytokine .The aim of this study is to investigate the serum level and polymorphisms of IL-13 gene in patients with H.pylori infection. Methods: In this case control study, DNA was isolated by salting out method from peripheral blood of 150 patients with H.pylori infection diagnosed by an expert. PCR-RFLP was used to examine polymorphisms of IL-13 gene . The results were compared with 150 healthy controls by SPSS software and Chi-square test.Tostudy, the serum levels of IL-13 cytokine, serum samples of 40 patients and 40 controls were prepared and examined using ELISA kit. Results: According to the findings, there is a significant difference in (CI: 06/1-09/0, OR 32.0 MB) GA (P = 0/005) and (08/1-52/0: CI, OR: 45.0) GG (P = 0/045) genotypes at A/2044 G +, position between patients and the control group. No significant differences were observed at the position of -1512 A/C. Also, no significant differences were seen between serum level of IL-13 cytokine and H. pylori disease (P >. 0.05). Conclusion: Based on the results obtained, it seems that GA and GG genotypes of interleukin-13 gene at position 2044+ make people more susceptible to H. pylori infection.
