HPLC-Analysis of Phenolic Complex in Pectoral Species № 4 and it’s Dry Extract

Abstract

Introduction. There is steady upward trend in demand for herbal formulation necessitates the expansion of their range. Recent studies have shown that one of the promising research direction is the development of herbal collection based water-soluble dry extracts. Pectoral species № 4 is one of the most popular among multi-component herbal preparations on the Russian pharmaceutical market. Experimental samples of a dry extract were obtained on the collection basis. According to literature, phenolic compounds predominate both in individual components and pectoral species № 4. Therefore, the end-to-end standardization of the collection and its dry extract by the content of the substances seems to be relevant.Aim. The present study was performed to examine the dry extract phenolic compounds composition, as well as, to search for marker compounds for the end-to-end standardization of pectoral species № 4 and dosage forms based on it.Materials and methods. The composition and content of phenolic complex were studied by HPLC with UV detection in pectoral species № 4 and its dry extract. Detection was performed at wavelengths of 350, 300, 270, 210 nm. The quantitative content (%) of the identified compounds was determined by an external standard method.Results and discussion. HPLC-analysis revealed 58 compounds in pectoral species № 4 and 34 in the extract. Rutin, arbutin, liquraside, liquiritin, quercetin, chlorogenic, caffeic and gallic acid have been identified in all study objects; their quantitative content varied within a range of 0,001−0,910%.Conclusion. Arbutin, rutin and chlorogenic acid have been proposed as marker compounds.

Keywords

• pectoral species
• dry extract
• phenolic compounds
• hplc
• standardization