Nature Communications (Dec 2016)
Detecting stoichiometry of macromolecular complexes in live cells using FRET
Abstract
Measuring thein vivo stoichiometry of protein-protein interactions is challenging. Here the authors take a FRET-based approach, quantifying stoichiometry based on ratiometric comparison of donor and acceptor fluorescence, and apply their method to report on a Ca2+-induced switch in calmodulin binding to Ca2+ion channels.