Nature Communications (Dec 2016)

Detecting stoichiometry of macromolecular complexes in live cells using FRET

  • Manu Ben-Johny,
  • Daniel N. Yue,
  • David T. Yue

DOI
https://doi.org/10.1038/ncomms13709
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 10

Abstract

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Measuring thein vivo stoichiometry of protein-protein interactions is challenging. Here the authors take a FRET-based approach, quantifying stoichiometry based on ratiometric comparison of donor and acceptor fluorescence, and apply their method to report on a Ca2+-induced switch in calmodulin binding to Ca2+ion channels.