Detecting stoichiometry of macromolecular complexes in live cells using FRET

Manu Ben-Johny; Daniel N. Yue; David T. Yue

doi:10.1038/ncomms13709

Nature Communications (Dec 2016)

Detecting stoichiometry of macromolecular complexes in live cells using FRET

Manu Ben-Johny,
Daniel N. Yue,
David T. Yue

Affiliations

Manu Ben-Johny: Department of Biomedical Engineering, Calcium Signals Laboratory, The Johns Hopkins University School of Medicine
Daniel N. Yue: Department of Biomedical Engineering, Calcium Signals Laboratory, The Johns Hopkins University School of Medicine
David T. Yue: Department of Biomedical Engineering, Calcium Signals Laboratory, The Johns Hopkins University School of Medicine

DOI: https://doi.org/10.1038/ncomms13709
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 10

Abstract

Read online

Measuring thein vivo stoichiometry of protein-protein interactions is challenging. Here the authors take a FRET-based approach, quantifying stoichiometry based on ratiometric comparison of donor and acceptor fluorescence, and apply their method to report on a Ca2+-induced switch in calmodulin binding to Ca2+ion channels.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal