Nature Communications (Apr 2020)
Quantifying the heterogeneity of macromolecular machines by mass photometry
- Adar Sonn-Segev,
- Katarina Belacic,
- Tatyana Bodrug,
- Gavin Young,
- Ryan T. VanderLinden,
- Brenda A. Schulman,
- Johannes Schimpf,
- Thorsten Friedrich,
- Phat Vinh Dip,
- Thomas U. Schwartz,
- Benedikt Bauer,
- Jan-Michael Peters,
- Weston B. Struwe,
- Justin L. P. Benesch,
- Nicholas G. Brown,
- David Haselbach,
- Philipp Kukura
Affiliations
- Adar Sonn-Segev
- Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford
- Katarina Belacic
- Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC)
- Tatyana Bodrug
- Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill
- Gavin Young
- Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford
- Ryan T. VanderLinden
- Department of Structural Biology, St. Jude Children’s Research Hospital
- Brenda A. Schulman
- Department of Structural Biology, St. Jude Children’s Research Hospital
- Johannes Schimpf
- Albert-Ludwigs-Universität, Institut für Biochemie, Albertstr. 21, Chemie-Hochhaus
- Thorsten Friedrich
- Albert-Ludwigs-Universität, Institut für Biochemie, Albertstr. 21, Chemie-Hochhaus
- Phat Vinh Dip
- Department of Biology, Massachusetts Institute of Technology
- Thomas U. Schwartz
- Department of Biology, Massachusetts Institute of Technology
- Benedikt Bauer
- Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC)
- Jan-Michael Peters
- Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC)
- Weston B. Struwe
- Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford
- Justin L. P. Benesch
- Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford
- Nicholas G. Brown
- Department of Pharmacology and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine
- David Haselbach
- Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC)
- Philipp Kukura
- Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford
- DOI
- https://doi.org/10.1038/s41467-020-15642-w
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 10
Abstract
Mass photometry is a label-free optical approach capable of detecting, imaging and accurately measuring the mass of single biomolecules in solution. Here, the authors demonstrate the potential of mass photometry for quantitatively characterizing sample heterogeneity of purified protein complexes with implications for structural studies specifically and in vitro studies more generally.
