Chloroacetanilides inhibit photosynthesis and disrupt the thylakoid membranes of the dinoflagellate Prorocentrum minimum as revealed with metazachlor treatment

Abstract

The chloroacetanilides are among the most commonly used herbicides worldwide, which contaminate aquatic environments and affect aquatic phototrophs. Their sub-lethal toxicity has been evaluated using freshwater algae; however, the modes of cellular toxicity and levels of toxicity to marine organisms are not fully understood. In the present study, we assessed the cellular and molecular effects of chloroacetanilides on marine phototrophs using the dinoflagellate Prorocentrum minimum and the herbicide metazachlor (MZC). The MZC treatment led to a considerable reduction in cell number and pigment, and the EC50 of MZC was calculated to be 0.647 mg/L. The photosynthetic parameters, Fv/Fm and chlorophyll fluorescence significantly decreased with MZC exposure time in a dose-dependent manner. In addition, MZC significantly induced photosynthesis genes, including PmpsbA, PmpsaA, and PmatpB, and the antioxidant PmGST, but not PmKatG. These findings were well matched to reactive oxygen species (ROS) production in MZC-treated cells. Interestingly, we observed inflated vacuoles, undivided chloroplasts, and breakdown of thylakoid membranes in MZC-treated cells. These results support the hypothesis that MZC severely damages chloroplasts, resulting in dysfunction of the dinoflagellate photosynthesis and possibly marine phototrophs in the environment.

Keywords

• Metazachlor
• Prorocentrum minimum
• Photosynthesis inhibition
• Gene expression
• Oxidative stress