EXPRESSION OF P53, P27kip1AND Ki-67 IN GASTRIC CANCER AND THEIR CORRELATION WITH CLINICOHISTOPATHOLOGIC PARAMETERS IN SHAHID BEHESHTI HOSPITAL (BABOL 2000-2006)

Abstract

BACKGROUND AND OBJECTIVE: Different studies have reported different results for expression of cell-cycle regulator genes, P53 and P27kip1, and proliferation marker Ki-67 in gastric cancer and their correlation with clinicohistopathologic parameters and outcome. The aim of this study was to study the expression rate of P53,P27kip1, Ki-67 and their correlation with histopathological features and subsequently biological behavior in gastric cancer. METHODS: In this experimental (laboratory) study, 58 paraffin-embedded blocks from patients who underwent gastrectomy were stained by hematoxylin-eosin and then immunohistochemistry against P53, P27kip1 and Ki-67. The statistical associations between clinicopathological parameters and immunohistochemical results were tested by Chi- Square and Fishers- exact test using SPSS. FINDINGS: Male: female=2.86:1 median age= 60, antral location (27 cases), ulcerating macroscopic appearance (29 cases), intestinal (40 cases) and infiltrative type (48 cases) were the most frequent findings. Histological grade G1 (differentiated, 29 cases) and T3 + T4 stages (49 cases) were the most frequent. Number of cases with lymph node metastasis, vascular and neural invasion was 41, 49 and 47, respectively. Expression rate of P53, P27Kip1 and Ki-67 were 56.9%, 56.9% and 75.9%, respectively. There was only a significant correlation between expression of P53 and Ki-67 (p= 0.028, r=0.323).CONCLUSION: According to the results, studying the expression of P53, P27kip1 and Ki-67 markers alone do not have diagnostic value in predicting the biological behavior of gastric cancer.

Keywords

• gastric cancer
• p53
• p27
• ki-67
• immunohistochemistry