FEATURES OF LUMINOL- AND LUCIGENIN-INDUCED CHEMILUMINESCENCE OF NEUTROPHILIC GRANULOCYTES IN PATIENTS WITH CHRONIC RHINOSINUSITIS

Abstract

Studies of functional activity of neutrophilic granulocytes from patients with chronic rhinosinusitis was performed using luminol- or lucigenin-dependent chemiluminescence. We have revealed increased production of reactive oxygen species (ROS) both under basal conditions, and in zymozan-induced chemiluminescent reaction, employing luminol as an activator, and determining total functional activity of neutrophils. Meanwhile, in lucigenin-induced chemiluminescence associated with production of superoxide anion radicals, a delayed ROS production was revealed, both in spontaneous and zymozan-induced response. One may suggest that, when studying these pathological events in neutrophils, ROS production in the cells proceeds mostly by myeloperoxidase pathway.

Keywords

• neutrophilic granulocytes
• luminol
• lucigenin
• reactive oxygen species
• rhinosinusitis